AEC, 50X (CAS 132-32-1)

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Synonym:	3-amino-9-ethylcarbazole
Application:	(3-amino-9-ethylcarbazole); a frequently used precipitable peroxidase substrate for immunohistochemical staining
CAS Number:	132-32-1
Molecular Weight:	210.3
Molecular Formula:	C₁₄H₁₄N₂

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Description

AEC (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X; sc-24982), the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; sc-24943). Use with AEC Substrate Buffer (sc-36873).

Usage

• Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.
• Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X; sc-36873).
• Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.
• Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.
• Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.
• Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; sc-24943)
• Unused AEC solution can be discarded according to city, county, state, province or country’s regulations.

Technical Information

Appearance:	red
Physical State:	Solid
Solubility:	Soluble in acetone (50 mg/ml), and DMF. Insoluble in water.
Storage:	Store at 4° C
Melting Point:	98-100 °C
Boiling Point:	420.90 °C at 760 mmHg
Density:	1.17 g/cm³
Refractive Index:	n²⁰_D 1.65
IC50:	PhoP regulon: EC₅₀50 = 16.14 µM (Salmonella typhimurium); oncogenic and cytokinetic protein MgcRacGAP: IC₅₀ = 64.1 µM (human)

Safety and Reference Information

Transport:	UN 2810, Class 6.1, Packing group III
WGK Germany:	3
RTECS:	FE3590000
PubChem CID:	8588
MDL Number:	MFCD00004964
EC Number:	205-057-7
Beilstein Registry:	174969
SMILES:	CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Graham, R.C., et al. 1965. J. Histochem. Cytochem. 13: 150-152. PMID: 14293042
2. Krenács, T., et al. 1990. Histochem. J. 22: 530-536. PMID: 1705250
3. Okano, K., et al. 1992. Rinsho Byori. 40: 93-97. PMID: 1372054
4. Galey, S., et al. 2003. Orthopedics. 26: 1201-1204. PMID: 14690289

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