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Bromophenol Blue (CAS 115-39-9)

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Synonym:3',3'',5',5''-Tetrabromophenolsulfonephthalein
Application:An electrophoresis tracking dye also used as an acid-base indicator
CAS Number:115-39-9
Grade:ACS
Molecular Weight:669.96
Molecular Formula:C19H10Br4O5S
Refer to Certificate of Analysis for lot specific data (including water content).
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Bromophenol Blue sc-24971 25 g $68
Bromophenol Blue sc-24971A 100 g $210
Description
Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis it can be used as a color marker and as as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.
Technical Information
Physical State:Solid
Solubility:Freely soluble in NaOH. More soluble in methy and ethyl alcohols ,benzene, and in acetic acid.Slightly soluble in water (0.4g/100g) at 20 °C.
Storage:Store at room temperature
Melting Point:273 °C
Boiling Point:605.61 °C at 760 mmHg
Density:2.2 g/cm3
Refractive Index:n20D 1.74
IC50:HIV-1(RF): EC5050 = >50 µM (CEM-SS ); HIV-1(RF): IC50 = >50 µM (CEM-SS )
pK Values:pKa: 4.0
Safety and Reference Information
WGK Germany:3
RTECS:SJ7453000
PubChem CID:8272
Merck Index:14: 1444
MDL Number:MFCD00005875
EC Number:204-086-2
Beilstein Registry:61698
SMILES:C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
1. Menzies, D.W. 1961. Stain Technol. 36: 285-287. PMID: 14472719
2. Flores, R., et al. 1978. Anal. Biochem. 88: 605-611. PMID: 697029
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