EGF treated A-431 cells were processed by using the PhosphoCruz™ Protein Purification System sc-24964. Fig. 1: Blots were probed with phosphotyrosine antibody, sc-508, detecting tyrosine phosphorylated EGFR. Lanes A/B: unbound column fractions. Lanes C/D: bound column (phosphoprotein enriched) fractions. Lanes E/F: whole cell lysates prepared from untreated and EGF-treated cultures, respectively. Fig. 2: The same samples shown in Fig. 1 were analyzed with antibody to HSP 60, sc-13115.
PhosphoCruz™ Protein Purification System: sc-24964
- affinity purification system devised to enrich/isolate phosphoproteins from cell lysates and tissue extracts for phosphorylation studies; proteins that carry a phosphate group are retained on the column while non-phosphorylated and undesired contaminants pass through
- typical phosphoenriched protein yield is 5-10% of total protein
- contains materials required for six individual phosphoprotein enrichment procedures including six single-use columns, 100 ml 2X Binding/Wash Buffer, 30 ml Eluting Buffer, 150 µl each Phosphatase Inhibitor Cocktails 1 and 2, and six ultrafiltration concentrators for concentrating phosphoenriched protein post-purification
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PhosphoCruz™ Protein Purification System References:
- High copy amplification of the Aurora-A gene is associated with chromosomal instability phenotype in human colorectal cancers. | Nishida, N., et al. 2007. Cancer Biol Ther. 6: 525-33. PMID: 17457043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PhosphoCruz™ Protein Purification System | sc-24964 | 6 purifications | $295.00 |