Date published: 2025-9-7

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Calyculin A (CAS 101932-71-2)

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Application:
Calyculin A is a cell-permeable inhibitor of phosphatase 1 (PP1) and protein phosphatase 2A (PP2A-C), and may promote tumor growth
CAS Number:
101932-71-2
Purity:
≥98%
Molecular Weight:
1009.17
Molecular Formula:
C50H81N4O15P
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Calyculin A, a marine toxin found in Disodermia calyx, is a structurally distinct inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A-C). It has been shown that calyculin A displays inhibitory properties towards the endogenous phosphatase of smooth muscle myosin B and induces contraction in the muscle fibers. Contraction induced by calyculin A was accompanied by an increase in the cytosolic free Ca2+ concentration. Additionally it has been reported that this compound can bind to protein phosphatases in mouse skin, which serve as okadaic acid receptors. Consequentially, calyculin A induces ornithine decarboxylase in the mouse skin and is believed to promote tumor growth.


Calyculin A (CAS 101932-71-2) References

  1. Calyculin-A induces focal adhesion assembly and tyrosine phosphorylation of p125(Fak), p130(Cas), and paxillin in Swiss 3T3 cells.  |  Leopoldt, D., et al. 2001. J Cell Physiol. 188: 106-19. PMID: 11382927
  2. Crystal structure of the complex between calyculin A and the catalytic subunit of protein phosphatase 1.  |  Kita, A., et al. 2002. Structure. 10: 715-24. PMID: 12015153
  3. Calyculin-A increases the level of protein phosphorylation and changes the shape of 3T3 fibroblasts.  |  Chartier, L., et al. 1991. Cell Motil Cytoskeleton. 18: 26-40. PMID: 1848484
  4. Calyculin-A improves chromosome condensation for cytogenetic analysis of blastomeres from bovine and murine eight-cell stage embryos.  |  Kramer, JM., et al. 2008. Theriogenology. 70: 692-703. PMID: 18556054
  5. Mechanism of activation of PKB/Akt by the protein phosphatase inhibitor Calyculin A.  |  Pozuelo-Rubio, M., et al. 2010. Cell Biochem Biophys. 58: 147-56. PMID: 20717744
  6. Berberine attenuates calyculin A-induced cytotoxicity and Tau hyperphosphorylation in HEK293 cells.  |  Yu, G., et al. 2011. J Alzheimers Dis. 24: 525-35. PMID: 21297267
  7. Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin.  |  Suganuma, M., et al. 1990. Cancer Res. 50: 3521-5. PMID: 2160320
  8. The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells.  |  Edelson, JR. and Brautigan, DL. 2011. Toxins (Basel). 3: 105-19. PMID: 22069692
  9. Calyculin A from Discodermia calyx is a dual action toxin that blocks calcium influx and inhibits protein Ser/Thr phosphatases.  |  Holy, M. and Brautigan, DL. 2012. Toxins (Basel). 4: 940-54. PMID: 23162706
  10. Calyculin biogenesis from a pyrophosphate protoxin produced by a sponge symbiont.  |  Wakimoto, T., et al. 2014. Nat Chem Biol. 10: 648-55. PMID: 24974231
  11. Calyculin A and okadaic acid: inhibitors of protein phosphatase activity.  |  Ishihara, H., et al. 1989. Biochem Biophys Res Commun. 159: 871-7. PMID: 2539153
  12. Calcium-independent activation of contractile apparatus in smooth muscle by calyculin-A.  |  Ishihara, H., et al. 1989. J Pharmacol Exp Ther. 250: 388-96. PMID: 2545866
  13. Stimulation of Suicidal Erythrocyte Death by Phosphatase Inhibitor Calyculin A.  |  Almasry, M., et al. 2016. Cell Physiol Biochem. 40: 163-171. PMID: 27855413
  14. The protein phosphatase inhibitor calyculin-A affects catecholamine secretion and granular distribution in cultured adrenomedullary chromaffin cells.  |  Gutierrez, LM., et al. 1995. Eur J Cell Biol. 68: 88-95. PMID: 8549595
  15. Inhibition of protein phosphatase activity and changes in protein phosphorylation following acetaminophen exposure in cultured mouse hepatocytes.  |  Bruno, MK., et al. 1998. Toxicol Appl Pharmacol. 153: 119-32. PMID: 9875306

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Calyculin A, 10 µg

sc-24000
10 µg
$160.00

Calyculin A, 100 µg

sc-24000A
100 µg
$750.00

Calyculin A, 500 µg

sc-24000B
500 µg
$1400.00

Calyculin A, 1 mg

sc-24000C
1 mg
$3000.00

Can I store calyculin A at -80? How many microliters should I prepare my aliquots?

Asked by: cahide
Thank you for your question. We always recommend using solutions the same day they are made, however, solutions should be stable for up to 3 months at -20ºC.
Answered by: BlakeJ
Date published: 2025-01-02

Due to the small quantity, what is the best way to recover the sample from the vial?

Asked by: SCchem16
Thank you for your question. This chemical, Calyculin A (CAS 101932-71-2): sc-24000, is soluble in DMSO and ethanol. Try dissolving it in either of these solvents to get the sample out.
Answered by: Technical Support
Date published: 2019-09-18
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Rated 5 out of 5 by from Worked as expectedUsed 50 uM for 60 minutes, worked great in my cells, was able to reproduce past data using this compound for the first time
Date published: 2023-01-23
Rated 5 out of 5 by from Product scProduct sc-24000 (50nM, 20 minutes incubation) was used successfully to analyze prematurely condensed chromosomes (PCC). Mouse MEF cell lines were utilized (PubMed ID 20813759). -SCBT Publication Review
Date published: 2015-03-04
Rated 5 out of 5 by from To determine if Ser/Thr protein phosphatasesTo determine if Ser/Thr protein phosphatases such as PP2A serve an important role in the mTOR signaling pathway in mammalian cells, Calyculin A (SCBT product # sc-24000) was used to pre-treat PC-3 cells, followed by C-K BA treatment.
Date published: 2015-03-04
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Calyculin A is rated 5.0 out of 5 by 3.
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