CREB1 Antibody (X-12): sc-240
- CREB1 Antibody (X-12) is a mouse monoclonal IgG1 κ, cited in 92 publications, provided at 200 µg/ml
- raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1
- Anti-CREB1 Antibody (X-12) is recommended for detection of CREB-1A and CREB-1B of mouse, rat and human origin by WB, IP and IF
- Anti-CREB1 Antibody (X-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-240 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CREB1 (X-12): sc-240.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CREB1 Antibody (X-12) for WB applications. These reagents are now offered in bundles with CREB1 Antibody (X-12) (see ordering information below).
QUICK LINKS
CREB-1 Antibody (X-12) is a mouse monoclonal IgG1 kappa light chain antibody specifically targeting amino acids 254-327 within the DNA binding and dimerization domain of human CREB-1, enabling precise detection of CREB-1A and CREB-1B isoforms in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) assays. CREB-1 monoclonal antibody (X-12) recognizes cAMP response element-binding protein 1, also known as CREB and CREB1, a pivotal transcription factor predominantly localized in the cell nucleus, where binding occurs to cAMP response elements (CRE) in target gene promoters, regulating critical cellular processes such as metabolism, neuronal plasticity, and cell survival. Nuclear localization of CREB-1 (X-12) is crucial for modulating gene expression in response to various extracellular signals, including hormonal and stress stimuli. Upon phosphorylation at Serine 133 by protein kinase A, CREB-1 undergoes a conformational change enhancing interaction with CREB-binding protein (CBP), facilitating basal transcription complex assembly and activating transcriptional programs essential for cellular adaptation and function. Dynamic localization and activation mechanisms underscore CREB-1′s significance in neoglucogenesis through interaction with nuclear coactivator PGC-1, as well as involvement in type II diabetes and dilated cardiomyopathy pathogenesis, highlighting vital roles in metabolic regulation and cardiovascular health. Anti-CREB-1 monoclonal antibody (X-12) is available in non-conjugated form and various conjugated forms including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates, providing versatile options to meet experimental requirements. CREB-1 (X-12) antibody is recommended for investigating intricate pathways governed by CREB-1 and impact on cellular function and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREB1 Antibody (X-12) | sc-240 | 200 µg/ml | $316.00 | |||
CREB1 Antibody (X-12): m-IgG Fc BP-HRP Bundle | sc-526451 | 200 µg Ab; 10 µg BP | $354.00 | |||
CREB1 Antibody (X-12): m-IgG1 BP-HRP Bundle | sc-531824 | 200 µg Ab; 20 µg BP | $354.00 | |||
CREB1 Antibody (X-12) X | sc-240 X | 200 µg/0.1 ml | $316.00 | |||
CREB1 Antibody (X-12) AC | sc-240 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CREB1 Antibody (X-12) HRP | sc-240 HRP | 200 µg/ml | $316.00 | |||
CREB1 Antibody (X-12) FITC | sc-240 FITC | 200 µg/ml | $330.00 | |||
CREB1 Antibody (X-12) PE | sc-240 PE | 200 µg/ml | $343.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 488 | sc-240 AF488 | 200 µg/ml | $357.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 546 | sc-240 AF546 | 200 µg/ml | $357.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 594 | sc-240 AF594 | 200 µg/ml | $357.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 647 | sc-240 AF647 | 200 µg/ml | $357.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 680 | sc-240 AF680 | 200 µg/ml | $357.00 | |||
CREB1 Antibody (X-12) Alexa Fluor® 790 | sc-240 AF790 | 200 µg/ml | $357.00 |