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acetylated α Tubulin Antibody (6-11B-1): sc-23950

 |  Datasheet

(Based on data analysis)

  • acetylated α Tubulin Antibody (6-11B-1) is a mouse monoclonal IgG2b provided at 200 µg/ml
  • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
  • recommended for detection of acetylated α-tubulin of mammalian species, Drosophila, zebrafish and Xenopus origin by WB, IP and IF
  • See 13 citations for acetylated α Tubulin Antibody (6-11B-1)
 

See additional Tubulin Antibodies including Tubulin, α8 Tubulin, β1 Tubulin, β4 Tubulin, β5 Tubulin, β6 Tubulin, β8 Tubulin, alpha 1 Tubulin, alpha Tubulin, beta Tubulin, delta Tubulin, epsilon Tubulin, gamma Tubulin, beta 2 Tubulin, alpha 3 Tubulin, beta 3 Tubulin and alpha 4 Tubulin.

Also see chemical Tubulin Inhibitors for functional analysis of cellular responses to Tubulin.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
acetylated α Tubulin Antibody (6-11B-1) sc-23950 200 µg/ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
α1c Tubulin CRISPR/Cas9 KO Plasmid (m) sc-423551 20 µg $345
α1c Tubulin HDR Plasmid (m) sc-423551-HDR 20 µg $385
α1c Tubulin CRISPR/Cas9 KO Plasmid (h) sc-418230 20 µg $345
α1c Tubulin HDR Plasmid (h) sc-418230-HDR 20 µg $385
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
PC-12 Cell Lysate sc-2250 500 µg/200 µl $104
α1b Tubulin (m): 293T Lysate sc-118096 100 µg/200 µl $205
α8 Tubulin (h): 293T Lysate sc-127878 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human TUBA4A 7277 2q35 NM_006000 P68366
191110
Human TUBA3C 7278 13q12.11 NM_006001, NM_079836 Q13748
602528
Human TUBA1A 7846 12q13.12 NM_006009 Q71U36
611603
Human TUBA1B 10376 12q13.12 NM_006082 P68363
602530
Human TUBA8 51807 22q11.21 NM_018943 Q9NY65
605742
Human TUBA1C 84790 12q13.12 NM_032704 Q9BQE3
n/a
Human TUBA3E 112714 2q21.1 NM_207312 Q6PEY2
n/a
Human TUBA3D 113457 2q21.1 NM_080386 NP_525125
n/a
Mouse Tuba1a 22142 15 F1 P68369
N/A
Mouse Tuba1b 22143 15 F1 P05213
N/A
Mouse Tuba3a 22144 6 F3 P05214
N/A
Mouse Tuba4a 22145 1 C3 P68368
N/A
Mouse Tuba1c 22146 15 F1 P68373
N/A
Mouse Tuba3b 22147 6 G3 P05214
N/A
Mouse Tuba8 53857 6 F1 Q9JJZ2
N/A
Mouse EG434428 434428 9 E3.2 XP_486246
N/A
 


Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.

acetylated α Tubulin Antibody (6-11B-1) Product Citations
See how others have used acetylated α Tubulin Antibody (6-11B-1) and or acetylated α Tubulin Antibody (6-11B-1) conjugates.


13 total citations
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acetylated α Tubulin Antibody (6-11B-1) Data
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Western blot analysis of α Tubulin acetylation in untreated (A,C) and deacetylase inhibitor cocktail (sc-362323) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B) and α Tubulin (DM1A): sc-32293 (C,D).
acetylated α Tubulin (6-11B-1): sc-23950. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of acetylated α Tubulin expression in NIH/3T3 whole cell lysate.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α1b Tubulin expression in non-transfected: sc-117752 (A) and human α1b Tubulin transfected: sc-113442 (B) 293T whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α1a Tubulin expression in non-transfected 293T: sc-117752 (A), human α1a Tubulin transfected 293T: sc-116290 (B) and HeLa (C) whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α Tubulin expression in non-transfected: sc-117752 (A) and mouse α Tubulin transfected: sc-118096 (B) 293T whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α8 Tubulin expression in non-transfected 293T: sc-117752 (A), mouse α8 Tubulin transfected 293T: sc-118133 (B) and NIH/3T3 (C) whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α3C Tubulin expression in non-transfected: sc-117752 (A) and human α3C Tubulin transfected: sc-173307 (B) 293T whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α8 Tubulin expression in non-transfected: sc-117752 (A) and human α8 Tubulin transfected: sc-127878 (B) 293T whole cell lysates.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of α3C Tubulin expression in non-transfected: sc-117752 (A) and human α3C Tubulin transfected: sc-173019 (B) 293T whole cell lysates.
Septin 7 (H-120): sc-20620. Double immunofluorescent staining of methanol fixed human retinal pigment epithelial (RPE) cells using acetylated α Tubulin (6-11B-1): sc-23950 and donkey anti-mouse IgG-CFL 555: sc-362268 showing cilia staining (red immunofluorescence), Septin 7 (H-120): sc-20620 and donkey anti-rabbit IgG-FITC: sc-2090 showing base of cilia staining (green immunofluorescence) and Hoechst 33342: sc-391054 nuclear counterstain (blue immunofluorescence). Image kindly provided by Moshe Kim, Department of Cell Biology, Hospital for Sick Children (Toronto, Canada)
acetylated α Tubulin (6-11B-1): sc-23950. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
acetylated α Tubulin (6-11B-1): sc-23950. Western blot analysis of acetylated α Tubulin in untreated (A) and Trichostatin A (sc-3511) treated (B) NIH/3T3 whole cell lysates. Note upregulation of acetylated α Tubulin in lane B.
Western blot analysis of acetylated α Tubulin acetylation in untreated (A) and Oxamflatin (sc-205960) treated (B) HeLa whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B). Note acetylation of acetylated α Tubulin in lane B.
Western blot analysis of α Tubulin expression in untreated (A,C) and Panobinostat (sc-208148) treated (B,D) A549 whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B) and α Tubulin (B-7): sc-5286 (C,D).
Western blot analysis of α Tubulin acetylation in untreated (A) and CBHA (sc-205240) treated (B) A549 whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B) and α Tubulin (B-7): sc-5286 (C,D).
Western blot analysis of α Tubulin acetylation in untreated (A,C) and Scriptaid (sc-202807) treated (B,D) A549 whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B) and α Tubulin (B-7): sc-5286 (C,D).
acetylated α Tubulin (6-11B-1): sc-23950. Immunofluorescent staining of methanol fixed human retinal pigment epithelial (RPE) cells showing microtubule-like staining (red immunofluorescence) and Hoechst 33342: sc-391054 nuclear counterstain (blue immunofluorescence). Secondary antibody used was donkey anti-mouse IgG-CFL 555: sc-362268 . Image kindly provided by Moshe Kim, Department of Cell Biology, Hospital for Sick Children (Toronto, Canada)
acetylated α Tubulin (6-11B-1): sc-23950. Immunofluorescent staining of methanol fixed human retinal pigment epithelial (RPE) cells showing mitotic spindle staining (red immunofluorescence) and Hoechst 33342: sc-391054 nuclear counterstain (blue immunofluorescence). Secondary antibody used was donkey anti-mouse IgG-CFL 555: sc-362268 . Image kindly provided by Moshe Kim, Department of Cell Biology, Hospital for Sick Children (Toronto, Canada)
acetylated α Tubulin (6-11B-1): sc-23950. Immunofluorescent staining of methanol fixed, serum-starved human retinal pigment epithelial (RPE) cells showing cilia staining (red immunofluorescence) and Hoechst 33342: sc-391054 nuclear counterstain (blue immunofluorescence). Secondary antibody used was donkey anti-mouse IgG-CFL 555: sc-362268 . Image kindly provided by Moshe Kim, Department of Cell Biology, Hospital for Sick Children (Toronto, Canada)
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