Kaiso Antibody (6F8): sc-23871

Kaiso Antibody (6F8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Kaiso protein of mouse, rat, human, canine, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Kaiso (6F8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Kaiso, also known as ZNF-kaiso, is a member of the BTB/POZ family of zinc finger transcription factors, which play crucial roles in embryonic development and cancer progression. Kaiso is primarily localized in the nucleus and is expressed in key tissues such as the brain, eye, ear, branchial arches, and spinal cord, as well as in NIH3T3 cells. This transcriptional repressor contains an amino-terminal BTB-POZ domain that facilitates protein-protein interactions and three carboxy-terminal zinc finger domains that specifically bind to symmetrically methylated DNA sequences. The ability of Kaiso to recognize these modified DNA sequences is significant, as this recognition allows for gene expression regulation in response to epigenetic changes, thereby influencing developmental processes and tumorigenesis. Kaiso interacts with p120 catenin, further highlighting Kaiso′s role in cellular signaling pathways. The diverse applications of anti-Kaiso antibody (6F8) make this reagent an essential tool for researchers studying the intricate mechanisms of gene regulation and Kaiso′s implications in various biological contexts.

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Kaiso Antibody (6F8) References:

1. The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor.  |  Daniel, JM. and Reynolds, AB. 1999. Mol Cell Biol. 19: 3614-23. PMID: 10207085
2. The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor.  |  Prokhortchouk, A., et al. 2001. Genes Dev. 15: 1613-8. PMID: 11445535
3. Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding protein.  |  Daniel, JM., et al. 2001. Hybridoma. 20: 159-66. PMID: 11461664
4. [KAISO--a new member of the BTB/POZ family specifically bindsto methylated DNA sequences].  |  Prokhorchuk, AV., et al. 2001. Genetika. 37: 737-44. PMID: 11517759
5. Isolation and characterization of XKaiso, a transcriptional repressor that associates with the catenin Xp120(ctn) in Xenopus laevis.  |  Kim, SW., et al. 2002. J Biol Chem. 277: 8202-8. PMID: 11751886
6. Kaiso regulates Znf131-mediated transcriptional activation.  |  Donaldson, NS., et al. 2010. Exp Cell Res. 316: 1692-705. PMID: 20303951
7. Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells.  |  Robinson, SC., et al. 2017. Cell Commun Signal. 15: 24. PMID: 28637464
8. Kaiso Protein in the Regulation of Brain and Behavior.  |  Kulikova, EA. and Kulikov, AV. 2018. Curr Protein Pept Sci. 19: 692-698. PMID: 29086688
9. Kaiso is required for MTG16-dependent effects on colitis-associated carcinoma.  |  Short, SP., et al. 2019. Oncogene. 38: 5091-5106. PMID: 30858547
10. Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway.  |  Tong, W., et al. 2021. Int J Mol Med. 47: PMID: 33576467
11. Kaiso Regulates DNA Methylation Homeostasis.  |  Kaplun, D., et al. 2021. Int J Mol Sci. 22: PMID: 34299205
12. Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.  |  Tian, W., et al. 2022. Mol Oncol. 16: 3192-3209. PMID: 35851744

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Kaiso Antibody (6F8) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Kaiso Antibody (6F8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Kaiso Antibody (6F8) Specifications

Alternate Product Name: Kaiso

Product Clone Name: 6F8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-504

Epitope Target: Kaiso

Epitope Species: human

Product Reactivity/Detection: kaiso

Species Reactivity/Detection: mouse, rat, human, canine, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Kaiso Antibody (6F8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent