epitope corresponding to dually phosphorylated Tyr 239 and 240 of Shc of human origin
recommended for detection of Tyr 239 and 240 dually phosphorylated Shc of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
Shc Background Information The Shc gene encodes three widely expressed proteins which act as substrates for receptors and tyrosine kinases in signal transduction pathways. Growth factor receptors with tyrosine kinase activity phosphorylate and thus modulate the function of Shc. Specifically, the tyrosine phosphorylation of Shc residues 239/240 and 317 stimulates activation of Ras/MAPK via recruitment of the Grb2-Sos complex, with Shc binding Grb2. These residues are present in all Shc isoforms. In vitro, tyrosine residues 239/240 are phosphorylated by the tyrosine kinase Src, while stimulation of hematopoietic cells with interleukin 3 (IL-3) results in Shc phosphorylation, primarily on residues Tyr 239 and Tyr 317. Similarly, insulin and EGF stimulate the phosphorylation of Shc and the subsequent binding of Shc and Grb2. Shc has a role in insulin-induced mitogenesis by competing with IRS to bind to the insulin receptor. The human Shc gene maps to chromosome 1q21.3.
