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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of TFIIE-α of human origin
- recommended for detection of TFIIE-α p57 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-237 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-237 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
GTF2E1 |
2960 |
3q13.33 |
NM_005513 |
P29083
|
189962 |
| Mouse |
Gtf2e1 |
74197 |
16 B3 |
|
Q9D0D5
|
N/A |
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TFIIE-α Background Information
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits, a and b. The structure of TFIIE appears to be a heterotetramer (å2∫2); both subunits are required for optimal basal-level transcription.
| TFIIE-α (C-17) Product Citations |
See how others have used TFIIE-α (C-17): sc-237 antibody and or TFIIE-α (C-17) antibody conjugates.
16 total citations Loading citations.
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TFIIE-α (C-17)
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TFIIE-α (C-17): sc-237. Western blot analysis of TFIIE-α expression in NIH/3T3 nuclear extract.
TFIIE-α siRNA (h): sc-36651. Western blot analysis of TFIIE-α expression in non-transfected control (A) and TFIIE-α siRNA transfected (B) HeLa cells. Blot probed with TFIIE-α (C-17): sc-237. GAPDH (FL-335): sc-25778 used as specificity and loading control. TFIIE-α (C-17): sc-237. ChIP analysis of TFIIE-α recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
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