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Sodium citrate tribasic hydrate (CAS 6858-44-2)

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Synonym:Citric acid trisodium salt
Application:For use in the preparation of citrate buffer for antigen unmasking in IHC
CAS Number:6858-44-2
Purity:≥99%
Molecular Weight:258.07 (anhydrous)
Molecular Formula:C6H5Na3O7•xH2O
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Sodium citrate tribasic hydrate sc-236898 1 kg $60
Description
As a conjugate base of a weak acid, Sodium Citrate can perform as a biological buffering agent because it resists changes in pH. Citric acid is one of a series of compounds responsible for the physiological oxidation of fats, carbohydrates and proteins to carbon dioxide and water. Often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.
Additional forms available:
Citric Acid Monohydrate (sc-203327)
Citric Acid, Anhydrous (sc-211113)
Sodium Citrate, Dihydrate (sc-203383)
Citric Acid Trisodium Salt (sc-214745)
Sodium citrate monobasic (sc-215869)
Citrate Concentrated Solution (sc-294091)
Usage
• For antigen retrieval, prepare a solution of 10 mM sodium citrate buffer, pH 6.0.
Technical Information
Appearance:Crystalline solid
Physical State:Solid
Solubility:Soluble in water (51.62 g/l) at 20 °C.
Storage:Store at room temperature
Safety and Reference Information
WGK Germany:1
PubChem CID:71474
EC Number:200-675-3
Beilstein Registry:3819251
SMILES:C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.O.O.[Na+].[Na+].[Na+]
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
1. Vandemark, P.J. and Smith, P.F. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243
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