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p-VASP (Ser 157)-R Antibody: sc-23506-R |
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- rabbit polyclonal IgG, 200 µg/ml
- recommended for detection of Ser 157 phosphorylated VASP of mouse, rat and human origin by WB, IP, IF and ELISA
- blocking peptide, sc-23506 P
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VASP Background Information
The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. A distantly related protein, VASP (vasodilator-stimulated phosphoprotein), is involved in the maintenance of cytoarchitecture by interacting with actin-like filaments. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is also phosphorylated on serine 239 by cGMP-dependent protein kinase. |
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p-VASP (Ser 157)-R
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p-VASP (Ser 157)-R: sc-23506-R. Western blot analysis of VASP phosphorylation expression in non-transfected: sc-117752 (A) and human VASP transfected: sc-114829 (B) 293T whole cell lysates. p-VASP (Ser 157)-R: sc-23506-R. Western blot analysis of VASP phosphorylation in untreated non-transfected: sc-117752 (A), untreated human VASP transfected: sc-114829 (B) and lambda protein phosphatase treated human VASP transfected: sc-114829 (C) 293T whole cell lysates. p-VASP (Ser 157)-R: sc-23506-R. Western blot analysis of VASP phosphorylation in untreated (A) and lambda protein phosphatase treated (B) human platelet extract. p-VASP (Ser 157)-R: sc-23506-R. Western blot analysis of VASP phosphorlation in non-transfected: sc-117752 (A) and mouse VASP transfected: sc-127759 (B) 293T whole cell lysates and platelet extract (C). p-VASP (Ser 157)-R: sc-23506-R. Western blot analysis of VASP phosphorylation in non-transfected: sc-117752 (A) and mouse VASP transfected: sc-127758 (B) 293T whole cell lysates and human platelet extract (C). Western blot analysis of VASP phosphorylation in non-transfected: sc-117752 (A, D), untreated human VASP transfected: sc-114829 (B, E) and lambda protein phosphatase treated human VASP transfected: sc-114829 (C, F) 293T whole cell lysates. Antibodies tested include p-VASP (Ser 157)-R: sc-23506-R (A, B, C) and VASP (A-11): sc-46668 (D, E, F). Western blot analysis of VASP phosphorylation in untreated (A, C), and lambda protein phosphatase treated (B, D) human platelet extract. Antibodies tested include p-VASP (Ser 157)-R: sc-23506-R (A, B) and VASP (A-11): sc-46668 (C, D). |
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