TBS Blotto A: sc-2333

TBS Blotto A is a common blocking solution used in Western blotting and other immunoassay techniques. It is composed of Tris-buffered saline (TBS), typically containing Tris-HCl, NaCl, and sometimes Tween 20, along with a blocking agent such as non-fat dry milk or bovine serum albumin (BSA). In Western blotting, TBS Blotto A serves as a blocking solution to prevent non-specific binding of antibodies to the membrane and other proteins. The blocking agent (e.g., non-fat dry milk) blocks unoccupied binding sites on the membrane, reducing background noise and increasing the specificity and sensitivity of antibody detection. TBS Blotto A is added to the membrane after the primary antibody incubation step and before the secondary antibody incubation step in Western blotting protocols. It is incubated with the membrane for a period of time to ensure complete blocking of non-specific binding sites. Additionally, TBS Blotto A can be used as a diluent for primary and secondary antibodies, as well as for washing steps in immunoassay procedures. Its composition provides a suitable environment for antibody-antigen interactions and helps maintain the stability of proteins during the assay.

TBS Blotto A References:

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