epitope mapping near the C-terminus of Emi1 of human origin
recommended for detection of Emi1 of human and, to a lesser extent, mouse origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
Emi1 Background Information Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.
Emi1 (C-21)
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Emi1 (C-21): sc-23269. Western blot analysis of Emi1 expression in K-562 whole cell lysate.
Emi1 (C-21): sc-23269. Western blot analysis of Emi1 expression in non-transfected: sc-117752 (A) and mouse Emi1 transfected: sc-120019 (B) 293T whole cell lysates.
