Date published: 2025-9-23
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25-Hydroxyvitamin D2 (CAS 21343-40-8) - chemical structure image
Molecular structure of 25-Hydroxyvitamin D2, CAS Number: 21343-40-8
25-Hydroxyvitamin D2 (CAS 21343-40-8) - chemical structure image
Molecular structure of 25-Hydroxyvitamin D2, CAS Number: 21343-40-8
Molecular structure of 25-Hydroxyvitamin D2, CAS Number: 21343-40-8

25-Hydroxyvitamin D2 (CAS 21343-40-8)

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Alternate Names:
25-Hydroxyergocalciferol; 25-Hydroxycalciferol
Application:
25-Hydroxyvitamin D2 is biologically active vitamin D2 metabolite
CAS Number:
21343-40-8
Purity:
≥98%
Molecular Weight:
412.65
Molecular Formula:
C28H44O2
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Technical Information
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SDS & Certificate of Analysis

25-Hydroxyvitamin D2 is used in serum to measure the concentration of vitamin D produced by the skin. It is known that 25-Hydroxyvitamin D2 can be detected in human serum using high-performance liquid chromatography and ultraviolet detection. Vitamin D2, known as ergocalciferol, is a fat soluble secosteroid which regulates neuromuscular function, reduces inflammation and controls cell proliferation, differentiation and apoptosis. Furthermore, 25-Hydroxyvitamin D2 can be converted into calcitriol, which can act as a local cytokine.


25-Hydroxyvitamin D2 (CAS 21343-40-8) References

  1. Rapid and sensitive high-performance liquid chromatographic method for simultaneous determination of retinol, alpha-tocopherol, 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human plasma with photodiode-array ultraviolet detection.  |  Alvarez, JC. and De Mazancourt, P. 2001. J Chromatogr B Biomed Sci Appl. 755: 129-35. PMID: 11393696
  2. Associations of 25-hydroxyvitamin D2 and D3 with cardiovascular risk factors in childhood: cross-sectional findings from the Avon Longitudinal Study of Parents and Children.  |  Williams, DM., et al. 2012. J Clin Endocrinol Metab. 97: 1563-71. PMID: 22344194
  3. Standardization of measurements of 25-hydroxyvitamin D3 and D2.  |  Thienpont, LM., et al. 2012. Scand J Clin Lab Invest Suppl. 243: 41-9. PMID: 22536761
  4. UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1.  |  Yasuda, K., et al. 2013. Biochem Biophys Res Commun. 434: 311-5. PMID: 23548573
  5. Higher 25(OH)D2 is associated with lower 25(OH)D3 and 1,25(OH)2D3.  |  Swanson, CM., et al. 2014. J Clin Endocrinol Metab. 99: 2736-44. PMID: 24828488
  6. 25-Hydroxyvitamin D2 /D3 levels and factors associated with systemic inflammation and melanoma survival in the Leeds Melanoma Cohort.  |  Newton-Bishop, JA., et al. 2015. Int J Cancer. 136: 2890-9. PMID: 25403087
  7. Quantitation of 25-hydroxyvitamin D2 and D3 in serum and plasma by LCMS/MS.  |  Lee, D., et al. 2015. Bioanalysis. 7: 167-78. PMID: 25587834
  8. A candidate reference measurement procedure for quantifying serum concentrations of 25-hydroxyvitamin D₃ and 25-hydroxyvitamin D₂ using isotope-dilution liquid chromatography-tandem mass spectrometry.  |  Mineva, EM., et al. 2015. Anal Bioanal Chem. 407: 5615-24. PMID: 25967149
  9. 25-Hydroxyvitamin D isomerizes to pre-25-hydroxyvitamin D in solution: considerations for calibration in clinical measurements.  |  Bedner, M. and Lippa, KA. 2015. Anal Bioanal Chem. 407: 8079-86. PMID: 26359234
  10. Magnesium status and supplementation influence vitamin D status and metabolism: results from a randomized trial.  |  Dai, Q., et al. 2018. Am J Clin Nutr. 108: 1249-1258. PMID: 30541089
  11. Variation in plasma 25-hydroxyvitamin D2 and D3 in normal pregnancy with gestational age, sampling season, and complications: A longitudinal cohort study.  |  Orvik, AB., et al. 2020. PLoS One. 15: e0231657. PMID: 32302333
  12. Improved Recognition of 25-Hydroxyvitamin D2 by 2 Automated Immunoassays.  |  Geno, KA., et al. 2020. J Appl Lab Med. 5: 1287-1295. PMID: 32529210
  13. Measurement of plasma 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in population of patients with cardiovascular disease by UPLC-MS/MS method.  |  Abouzid, M., et al. 2020. J Chromatogr B Analyt Technol Biomed Life Sci. 1159: 122350. PMID: 32890769
  14. 25-hydroxyvitamin D is a predictor of COVID-19 severity of hospitalized patients.  |  Nguyen, NN., et al. 2022. PLoS One. 17: e0268038. PMID: 35503795
  15. Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid Chromatography-Tandem Mass Spectrometry.  |  Munar, A., et al. 2022. Methods Mol Biol. 2546: 539-544. PMID: 36127620

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

25-Hydroxyvitamin D2, 1 mg

sc-231277
1 mg
$212.00

25-Hydroxyvitamin D2, 10 mg

sc-231277A
10 mg
$826.00

25-Hydroxyvitamin D2, 50 mg

sc-231277B
50 mg
$2662.00

What is the shipping temperature for this product?

Asked by: hawkeye11
This chemical, 25-Hydroxyvitamin D2 (CAS 21343-40-8), ships ambient.
Answered by: Chemical Support 1
Date published: 2017-04-10

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. 25-Hydroxyvitamin D2 has an appearance of a white to off-white solid.
Answered by: Technical Support
Date published: 2017-02-23
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Rated 5 out of 5 by from Dabrowski et alDabrowski et al. (PubMed ID 26035242) found that 25-Hydroxycalciferol led to lower production of granulocytes and macrophages colony stimulating factor, interleukin 6, Tumor Necrosis Factor, and an increase in mRNA expression for Cathelicidin Antimicrobial Peptide (CAP) in pregnant women. -SCBT Publication Review
Date published: 2015-04-21
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25-Hydroxyvitamin D2 | CAS 21343-40-8 is rated 5.0 out of 5 by 1.
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