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ADH Antibody (N-19): sc-22865

 |  Datasheet

(Based on data analysis)

  • ADH Antibody (N-19) is a goat polyclonal IgG provided at 200 µg/ml
  • epitope mapping near the N-terminus of ADH of human origin
  • recommended for detection of ADHα, ADHβ, ADHγ and ADH4 of human origin and ADH1 of mouse and rat origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • blocking peptide, sc-22865 P
 

See additional ADH Antibodies including ADH, ADHα, ADHγ, ADH1, ADHbeta, ADH4, ADH5, ADH6, ADH7 and ADH8.

Also see chemical ADH Inhibitors or chemical ADH gamma Inhibitors for functional analysis of cellular responses to ADH.


Ordering InformationGene Info
Recommended Support Products:
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WB   IP   IF   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
ADH Antibody (N-19) sc-22865 200 µg/ml $279
ADH (N-19) P sc-22865 P
(peptide)
100 µg/0.5 ml $61
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ADHβ CRISPR/Cas9 KO Plasmid (h) sc-402141 20 µg $345
ADHβ HDR Plasmid (h) sc-402141-HDR 20 µg $385
ADH1 CRISPR/Cas9 KO Plasmid (h) sc-402260 20 µg $345
ADH1 HDR Plasmid (h) sc-402260-HDR 20 µg $385
ADHγ CRISPR/Cas9 KO Plasmid (h) sc-402261 20 µg $345
ADHγ HDR Plasmid (h) sc-402261-HDR 20 µg $385
ADH5 CRISPR/Cas9 KO Plasmid (h) sc-403859 20 µg $345
ADH5 HDR Plasmid (h) sc-403859-HDR 20 µg $385
ADH7 CRISPR/Cas9 KO Plasmid (h) sc-405066 20 µg $345
ADH7 HDR Plasmid (h) sc-405066-HDR 20 µg $385
ADH8 CRISPR/Cas9 KO Plasmid (h) sc-406475 20 µg $345
ADH8 HDR Plasmid (h) sc-406475-HDR 20 µg $385
ADH4 CRISPR/Cas9 KO Plasmid (h) sc-409145 20 µg $345
ADH4 HDR Plasmid (h) sc-409145-HDR 20 µg $385
ADH6 CRISPR/Cas9 KO Plasmid (h) sc-409146 20 µg $345
ADH6 HDR Plasmid (h) sc-409146-HDR 20 µg $385
ADH1 CRISPR/Cas9 KO Plasmid (m) sc-419005 20 µg $345
ADH1 HDR Plasmid (m) sc-419005-HDR 20 µg $385
ADH4 CRISPR/Cas9 KO Plasmid (m) sc-424111 20 µg $345
ADH4 HDR Plasmid (m) sc-424111-HDR 20 µg $385
ADH5 CRISPR/Cas9 KO Plasmid (m) sc-419007 20 µg $345
ADH5 HDR Plasmid (m) sc-419007-HDR 20 µg $385
ADH6A CRISPR/Cas9 KO Plasmid (m) sc-427304 20 µg $345
ADH6A HDR Plasmid (m) sc-427304-HDR 20 µg $385
ADH7 CRISPR/Cas9 KO Plasmid (m) sc-419006 20 µg $345
ADH7 HDR Plasmid (m) sc-419006-HDR 20 µg $385
ADH8 CRISPR/Cas9 KO Plasmid (m) sc-429272 20 µg $345
ADH8 HDR Plasmid (m) sc-429272-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ADH1 siRNA (m) sc-41437 10 µM $258
ADH1 (m)-PR sc-41437-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ADH1 shRNA Plasmid (m) sc-41437-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ADH1 shRNA (m) Lentiviral Particles sc-41437-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Hep G2 Cell Lysate sc-2227 500 µg/200 µl $104
ADHγ (h): 293T Lysate sc-111481 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ADH1A 124 4q23 NM_000667 P07327
103700
Human ADH1B 125 4q23 NM_000668 P00325
103780
Human ADH1C 126 4q23 NM_000669 P00326
103730
Human ADH4 127 4q23 NM_000670 P08319
103740
Human ADH5 128 4q23 NM_000671 P11766
103710
Human ADH6 130 4q23 NM_000672 P28332
103735
Mouse Adh1 11522 3 G3 P00329
N/A
 


The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products (1). Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism (1). Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster (1–4). The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood (5). The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers (5,6). With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes (5). The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract (5).

ADH Antibody (N-19) Data
Click on image to enlarge
ADH (N-19): sc-22865. Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
ADH (N-19): sc-22865. Western blot analysis of ADH expression in Hep G2 whole cell lysate.
ADH (N-19): sc-22865. Western blot analysis of ADHγ expression in non-transfected: sc-117752 (A) and human ADHγ transfected: sc-111481 (B) 293T whole cell lysates.
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