epitope corresponding to amino acids 21-170 mapping near the N-terminus of Gfi-1B of human origin
recommended for detection of Gfi-1B of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-22795 X, 200 µg/0.1 ml
Gfi-1B Background Information Growth factor independent 1 (Gfi-1) is a transcriptional repressor that spe-cifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi-1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transciptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the SOCS-1 and -3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.13, respectively.
Gfi-1B (H-150) Product Citations
See how others have used Gfi-1B (H-150): sc-22795 antibody and or Gfi-1B (H-150) antibody conjugates.
