CENP-E Background Information A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere Protein E, CENP-E, is a kinetochore motor protein that specifies kinetochore binding in mitosis. Both CENP-E and CENP-F are expressed during mitosis where they mediate the G(2) to M phase checkpoint. CENP-E is also expressed in high levels during meiosis I and meiosis II where it localizes to the fibrous corona and outer plate of kinetochores on the meiotic chromosomes. CENP-E colocalizes with hBUBR1, a BUB-related kinase until mid-anaphase. After the first polar body emission, CENP-E localizes to the spindle-midzone, separating from hBUBR1 after mid-anaphase.
CENP-E (H-300)
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CENP-E (H-300): sc-22790. Western blot analysis of CENP-E expression in untreated (A) and calyculin A-treated (B) A-431 whole cell lysates.
