Jurkat + Calyculin A Cell Lysate: sc-2277

Jurkat + Calyculin A Cell Lysate is produced from Jurkat cells, a human T lymphocyte cell line extensively employed in immunological research, which has been treated with Calyculin A, a potent inhibitor known to affect various signaling pathways, particularly those involving protein phosphatases 1 and 2A. This treatment is designed to induce phosphorylation events within the cells by preventing dephosphorylation, thus preserving the phosphorylated state of proteins at the time of cell lysis. The lysate contains a rich array of cellular components, including proteins, enzymes, RNA, and other biomolecules, encapsulating a snapshot of the cellular state under specific induced conditions. Researchers utilize this lysate to study the dynamics of protein phosphorylation and to understand the cellular behaviors in response to inhibition of phosphatase activity. Such studies are pivotal in elucidating the roles of phosphorylation in cell signaling, activation, proliferation, and apoptosis. Jurkat + Calyculin A Cell Lysate is particularly valuable in experiments looking to map kinase activity, explore signal transduction pathways, and identify critical nodes within those pathways that govern cell function. This lysate provides a crucial tool for researchers focused on the fundamental aspects of cell biology and signaling mechanisms.

Jurkat + Calyculin A Cell Lysate References:

1. Preparation and use of reverse protein microarrays.  |  Pin, E., et al. 2014. Curr Protoc Protein Sci. 75: 27.7.1-27.7.29. PMID: 24510676