epitope mapping near the N-terminus of RB1CC1 of human origin
recommended for detection of RB1CC1 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
RB1CC1 Background Information The protein RB1CC1 (retinoblastoma 1 (RB1)-inducible coiled-coil 1) is a key regulator of the tumor-suppressor gene RB1 (1,2,3). RB1CC1 is abundantly expressed in human musculoskeletal and cultured osteosarcoma cells in amounts closely correlated to RB1 expression (3). Both the RB1CC1 and RB1 genes are preferentially co-expressed and contribute to the maturation of human embryonic musculoskeletal cells (3). RB1CC1 is localized in the nucleus and may be a transcription factor indicated by the presence of its nuclear localization signal, leucine zipper motif and coiled-coil structure (a ubiquitous protein folding and assembly motif made of alpha-helices wrapped around each other to form a supercoil) (1,2,4). The RB1CC1 gene has been identified in a screen for genes involved in multi-drug resistance to anticancer agents and is frequently mutated in breast cancer, showing characteristics of a tumor-suppressor gene (1,5). In mouse, Rb1cc1 protein is highly expressed in heart and testis, with lower levels detected in lung and spleen (5).
