epitope mapping at the C-terminus of RelB of mouse origin
recommended for detection of RelB p68 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and bovine
blocking peptide, sc-226 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-226 X, 200 µg/0.1 ml
RelB Background Information The NF˚B transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NF˚B subunit. Rel B does not bind with high affinity to NFkB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NF˚B homodimers. However, Rel B/p50 heterodimers, in contrast to NF˚B heterodimers, transactivates transcription of promotors containing ˚B binding sites.
RelB (C-19) Product Citations
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RelB (C-19)
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RelB (C-19): sc-226. Western blot analysis of RelB p68 expression in KNRK nuclear extract.
ChIP analysis of cytokine gene promoter occupancy in TNFα-treated mouse embryonic fibroblasts. IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): sc-109, RelB (C-19): sc-226 and p300 (N-15): sc-584. Data kindly provided by A. Hoffmann.
RelB (C-19): sc-226. Western blot analysis of RelB expression in non-transfected: sc-117752 (A) and human RelB transfected: sc-114651 (B) 293T whole cell lysates.
RelB (C-19): sc-226. Western blot analysis of RelB expression in non-transfected 293T: sc-117752 (A), mouse RelB transfected 293T: sc-127459 (B) and KNRK2 (C) whole cell lysates.
RelB (C-19): sc-226. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
