D-myo-Inositol 1,4,5-trisphosphate (caged) trisodium salt

D-myo-Inositol 1,4,5-trisphosphate (caged) trisodium salt, commonly referred to as caged Ins(1,4,5)P3, is a modified form of inositol trisphosphate (Ins(1,4,5)P3) used in research to temporally and spatially control the release of Ins(1,4,5)P3 and study its cellular effects. The "caged" designation indicates that a photolabile protecting group is attached to the molecule, rendering it inactive until exposure to light removes the cage, liberating the active Ins(1,4,5)P3. This property allows researchers to precisely manipulate the timing and location of Ins(1,4,5)P3-mediated signaling events in vitro and in vivo. Upon photolysis, caged Ins(1,4,5)P3 releases active Ins(1,4,5)P3, which then binds to its receptors on the endoplasmic reticulum membrane, leading to calcium release and downstream cellular responses. This technique enables the investigation of spatiotemporal aspects of calcium signaling and its impact on cellular physiology and pathophysiology. Researchers utilize caged Ins(1,4,5)P3 in various experimental paradigms, including studies on neuronal signaling, synaptic plasticity, fertilization, and immune responses. By employing caged Ins(1,4,5)P3, scientists can uncover complex details of Ins(1,4,5)P3-mediated signaling pathways and their roles in diverse cellular processes, offering insights into fundamental biological mechanisms and potential targets for various diseases.