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- goat polyclonal IgG, 200 µg/ml
- epitope mapping near the C-terminus of RFC5 of human origin
- recommended for detection of RFC5 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and porcine
- blocking peptide, sc-21890 P
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Ordering Information
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RFC5 Background Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism (1,2). RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction (1,3). All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences (1). Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs (4). The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not (5). The human RFC5 gene maps to chromosome 12q24.2-q24.3 and encodes the RFC5 subunit (6). RFC5 and RAD24 are required for DNA damage checkpoint control in the budding yeast Saccharomyces cerevisiae (7). RFC5 is part of a mechanism transducing the DNA damage signal to the activation of the central transducer Rad53 (8). |
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RFC5 (K-15)
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RFC5 (K-15): sc-21890. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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