Date published: 2025-9-10

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Methylene Violet 3RAX (CAS 4569-86-2)

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Alternate Names:
N,N-Diethylphenosafranine
Application:
Methylene Violet 3RAX is a potentially useful dye for biochemical studies
CAS Number:
4569-86-2
Molecular Weight:
378.90
Molecular Formula:
C22H23ClN4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Methylene Violet 3RAX is a useful dye for proteomics research. Methylene Violet 3RAX is a synthetic compound extensively utilized in scientific research and laboratory experiments. This versatile quaternary ammonium salt serves as a reagent in a wide array of applications, including organic synthesis, spectroscopy, and chromatography. As a colorless solid, Methylene Violet 3RAX easily dissolves in both water and organic solvents, making it highly adaptable for various purposes within the laboratory setting. In scientific research, this compound plays a vital role as a catalyst in numerous organic compound reactions, such as amines, alcohols, and carboxylic acids. Moreover, it contributes to the synthesis of organic compounds. Its application extends further to include the creation of fluorescent dyes, proteins, and other molecules with fluorescent properties. The exact mechanism of action of Methylene Violet 3RAX remains somewhat elusive. However, it is believed to act as a proton acceptor, facilitating proton transfers between molecules. This, in turn, can lead to the formation of new molecules or the breakdown of existing ones. Additionally, the compound exhibits chelating properties, enabling it to bind with metals and other ions, thus aiding in their stabilization.


Methylene Violet 3RAX (CAS 4569-86-2) References

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  2. Analysis of methylene blue in human urine by capillary electrophoresis.  |  Borwitzky, H., et al. 2005. J Chromatogr B Analyt Technol Biomed Life Sci. 826: 244-51. PMID: 16182616
  3. Quantification of cationic anti-malaria agent methylene blue in different human biological matrices using cation exchange chromatography coupled to tandem mass spectrometry.  |  Burhenne, J., et al. 2008. J Chromatogr B Analyt Technol Biomed Life Sci. 863: 273-82. PMID: 18258499
  4. BTI1, an azoreductase with pH-dependent substrate specificity.  |  Johansson, HE., et al. 2011. Appl Environ Microbiol. 77: 4223-5. PMID: 21531830
  5. Bioconjugatable azo-based dark-quencher dyes: synthesis and application to protease-activatable far-red fluorescent probes.  |  Chevalier, A., et al. 2013. Chemistry. 19: 1686-99. PMID: 23255474
  6. Water soluble octa-functionalized POSS: all-click chemistry synthesis and efficient host-guest encapsulation.  |  Han, J., et al. 2014. Chem Commun (Camb). 50: 8712-4. PMID: 24964315
  7. The kinetics of inhibition of human acetylcholinesterase and butyrylcholinesterase by methylene violet 3RAX.  |  Onder, S., et al. 2019. Chem Biol Interact. 314: 108845. PMID: 31593690
  8. Targeted reclaiming cationic dyes from dyeing wastewater with a dithiocarbamate-functionalized material through selective adsorption and efficient desorption.  |  Liu, Y., et al. 2020. J Colloid Interface Sci. 579: 766-777. PMID: 32673853
  9. Inhibition of cholinesterases by safranin O: Integration of inhibition kinetics with molecular docking simulations.  |  Onder, S., et al. 2021. Arch Biochem Biophys. 698: 108728. PMID: 33345803
  10. Development of novel hydrogen sulfide depletion aided platform for photodynamic therapy with enhanced anticancer performance.  |  Jia, F., et al. 2023. J Photochem Photobiol B. 239: 112646. PMID: 36638557
  11. Phenazinium dyes methylene violet 3RAX and indoine blue bind to DNA by intercalation: Evidence from structural and thermodynamic studies  |  Ishita Saha, Gopinatha Suresh Kumar. 2013. Dyes and Pigments. 96: 81-91.
  12. Quantitative determination of methylene blue in environmental samples by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry: a green approach  |  Mohammad Rizwan Khana, Moonis Ali Khan*a, Zeid Abdullah Alothmana, Ibrahim Hotan Alsohaimia, Mu Naushada and Nora H. Al-Shaalanb. 2014. RSC Adv. 4: 34037-34044.
  13. Methylene violet 3RAX-conjugated porphyrin for photodynamic therapy: synthesis, DNA photocleavage, and cell study  |  Si Yao‡ ac, Yunman Zheng‡b, Lijun Jiangd, Chen Xied, Fengshou Wu*c, Chi Huang*b, Xiong Zhangc, Ka-Leung Wong*de, Zaoying Lib and Kai Wang*a. 2018. RSC Adv. 8: 4472-4477.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Methylene Violet 3RAX, 1 g

sc-218835
1 g
$33.00

Methylene Violet 3RAX, 5 g

sc-218835A
5 g
$112.00