 |
- goat polyclonal IgG, 200 µg/ml
- epitope mapping near the C-terminus of RFC3 of human origin
- recommended for detection of RFC3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-21883 P
|
|
| |
Ordering Information
Recommended Support Products:
(click button of application of choice)
RFC3 Background Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism (1,2). RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction (1,3). All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences (1). Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs (4). The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not (5). RFC3 is required not only for DNA replication, but also for replication and damage checkpoint controls, probably functioning as a checkpoint sensor (6). The human RFC3 gene maps to chromosome 13q12.3-q13 and encodes the RFC3 subunit (7). In Saccharomyces cerevisiae, purified RFC3 has an ATPase activity that is markedly stimulated by single-stranded DNA but not by double-stranded DNA or RNA (8). |
|
|
RFC3 (K-19)
Click on image to enlarge
|
|
RFC3 (K-19): sc-21883. Western blot analysis of RFC3 expression in MCF7 whole cell lysate.
|
|
Download
|
|
