epitope corresponding to phosphorylated Ser 1177 of NOS3 of human origin
recommended for detection of Ser 1177 phosphorylated NOS3 of mouse, rat and human origin by WB and IF; also reactive with additional species, including canine, bovine and porcine
NOS3 Background Information Nitric oxide (NO), produced by the endothelial NO synthase (NOS3), is a fundamental determinant of cardiovascular homeostasis that maintains system blood pressure, vascular remodeling and angiogenesis. NOS3 is stimulated, in a phosphatidylinositol 3-kinase (PI 3-kinase)-dependent fashion, by treatment of endothelial cells with insulin-like growth factor-1 and vascular endothelial growth factor (VEGF). The serine/ threonine protein kinase Akt/PKB is an important downstream target of PI 3-kinase, regulating VEGF-stimulated endothelial cell survival. NOS3 activation via phosphorylation of serine 1177 by Akt/PKB is necessary and sufficient for VEGF-mediated endothelial cell migration. Therefore, Akt/PKB can directly phosphorylate NOS3 on serine 1177, activating the enzyme and leading to NO production.
p-NOS3 (Ser 1177)-R Product Citations
See how others have used p-NOS3 (Ser 1177)-R: sc-21871-R antibody and or p-NOS3 (Ser 1177)-R antibody conjugates.
