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- mouse monoclonal IgG1; 200 µg/ml
- raised against full length recombinant murine T-bet
- recommended for detection of T-bet of mouse and human origin by WB, IP, IF, FCM and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications, T-bet (4B10) X: sc-21749 X, 200 µg/0.1 ml
- fluorescein (sc-21749 FITC), phycoerythrin (sc-21749 PE), PerCP (sc-21749 PerCP) and PerCP-Cy5.5 (sc-21749 PCPC5) conjugates for intracellular FCM, 100 tests
- agarose conjugate for IP studies, T-bet (4B10) AC: sc-21749 AC, 500 µg/0.25 ml agarose
- Alexa Fluor® 405 (sc-21749 AF405), Alexa Fluor® 488 (sc-21749 AF488) and Alexa Fluor® 647 (sc-21749 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
TBX21 |
30009 |
17q21.32 |
NM_013351 |
Q9UL17
|
604895 |
| Mouse |
Tbx21 |
57765 |
11 D |
|
Q9JKD8
|
N/A |
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T-bet Background Information
T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-©. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.
| T-bet (4B10) Product Citations |
See how others have used T-bet (4B10): sc-21749 antibody and or T-bet (4B10) antibody conjugates.
44 total citations Loading citations.
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T-bet (4B10)
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T-bet (4B10): sc-21749. Western blot analysis of T-bet expression in BJAB nuclear extract.
T-bet (4B10) FITC: sc-21749 FITC. Intracellular FCM analysis of fixed and permeabilized human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2855. T-bet (4B10) PE: sc-21749 PE. Intracellular FCM analysis of fixed and permeabilized human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. T-bet (4B10): sc-21749. Western blot analysis of T-bet expression in BJAB whole cell lysate.
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