MMP-3 Antibody (1B4): sc-21732

MMP-3 Antibody (1B4) is a mouse monoclonal IgG1 antibody that detects MMP-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MMP-3 antibody (1B4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The matrix metalloproteinases (MMPs) are a crucial family of peptidase enzymes that play a significant role in the degradation of extracellular matrix components, such as collagen, gelatin, fibronectin, laminin, and proteoglycans, which are vital for tissue remodeling and repair. MMP-3, also known as stromelysin-1, is particularly important as MMP-3 not only activates procollagenase but also participates in the processing of other MMPs, thereby influencing various physiological and pathological processes, including wound healing, inflammation, and cancer progression. MMP-3 activation is tightly regulated and can occur through two distinct pathways: a slower direct activation by MMP-3 itself and a more rapid activation in conjunction with tissue or plasma proteinases. Understanding MMP-3 function is essential, as MMP-3 dysregulation is implicated in numerous diseases, including arthritis and cancer, making MMP-3 a valuable target for therapeutic intervention and research.

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MMP-3 Antibody (1B4) References:

1. A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas.  |  Basset, P., et al. Nature. 348: 699-704. PMID: 1701851
2. Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin).  |  Suzuki, K., et al. 1990. Biochemistry. 29: 10261-70. PMID: 2176865
3. The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin.  |  Saus, J., et al. 1988. J Biol Chem. 263: 6742-5. PMID: 3360803
4. Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma.  |  O'Callaghan, J., et al. 2023. Sci Adv. 9: eadf6537. PMID: 37075118
5. MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.  |  Zhang, Y., et al. 2023. Part Fibre Toxicol. 20: 22. PMID: 37217992
6. MMP-3 Knockout Induces Global Transcriptional Changes and Reduces Cerebral Infarction in Both Male and Female Models of Ischemic Stroke.  |  Hamblin, MH., et al. 2024. Int J Mol Sci. 25: PMID: 39000490
7. MMP-3 mediates copper oxide nanoparticle-induced pulmonary inflammation and fibrosis.  |  Zhang, Y., et al. 2024. J Nanobiotechnology. 22: 428. PMID: 39030581
8. Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
9. Matrix metalloproteinases: a review.  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
10. Activation of human neutrophil procollagenase by stromelysin 2.  |  Knäuper, V., et al. 1996. Eur J Biochem. 235: 187-91. PMID: 8631328
11. Expression of several matrix metalloproteinase genes in human monocytic cells.  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704
12. cDNA cloning and expression of the gene encoding murine stromelysin-2 (MMP-10).  |  Madlener, M. and Werner, S. 1997. Gene. 202: 75-81. PMID: 9427548

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP-3 Antibody (1B4) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP-3 Antibody (1B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP-3 Antibody (1B4) Specifications

Alternate Product Name: MMP-3

Product Clone Name: 1B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 317-327

Epitope Target: MMP-3

Epitope Species: human

Product Reactivity/Detection: MMP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for MMP-3 Antibody (1B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin