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p-eEF2K Antibody (Ser 366): sc-21645

 |  Datasheet

(Based on data analysis)
  • p-eEF2K Antibody (Ser 366) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-21645-R
  • recommended for detection of Ser 366 phosphorylated eEF2K of mouse, rat and human origin by WB and IF
  • blocking peptide, sc-21645 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
 

See additional eEF2K Antibodies.

Also see eEF2K Inhibitors for functional analysis of cellular responses to eEF2K.


Ordering InformationGene Info
Recommended Support Products:
(click button of application of choice)
WB   IF   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-eEF2K Antibody (Ser 366) sc-21645 200 µg/ml $279
p-eEF2K (Ser 366) P sc-21645 P
(peptide)
 100 µg/0.5 ml $61
p-eEF2K Antibody (Ser 366)-R sc-21645-R 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eEF2K siRNA (h) sc-39011 10 µM $258
eEF2K siRNA (m) sc-39012 10 µM $258
eEF2K (h)-PR sc-39011-PR 10 µM, 20 µl $23
eEF2K (m)-PR sc-39012-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eEF2K shRNA Plasmid (h) sc-39011-SH 20 µg $520
eEF2K shRNA Plasmid (m) sc-39012-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eEF2K shRNA (h) Lentiviral Particles sc-39011-V 200 µl $625
eEF2K shRNA (m) Lentiviral Particles sc-39012-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa + serum-starved + PMA Cell Lysate sc-24695 500 µg/200 µl $104
eEF2K (m): 293T Lysate sc-126762 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EEF2K 29904 16p12.1 NM_013302 O00418
 606968
Mouse Eef2k 13631 7 F2 O08796
 N/A
 

The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.