Trypan Blue (CAS 72-57-1)

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Synonym:	Direct Blue 14; Dianil blue; Diaminine Blue
Application:	Widely used in cell culture applications as a viability dye for quantifying live versus dead cells
CAS Number:	72-57-1
Molecular Weight:	960.81
Molecular Formula:	C₃₄H₂₄N₆O₁₄S₄Na₄

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
Trypan Blue	sc-216028	25 g	$65
Trypan Blue	sc-216028A	100 g	$215

Description

Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.

Technical Information

Appearance:	dark green to brown to brown black powder
Physical State:	Solid
Storage:	Store at 4° C
Color Index Number:	23850

Safety and Reference Information

PubChem CID:	9562061
MDL Number:	MFCD00003969
EC Number:	200-786-7
SMILES:	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC(=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O )(=O)[O-])C)NN=C5C(=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[ Na+].[Na+].[Na+]

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Bonnefille, P. et al. 2010. Fundam Clin Pharmacol. PMID: 21121944
2. Han, T.H. et al. 2010. J Acupunct Meridian Stud. 3(4): 241-248. PMID: 21185538
3. Saadatnia, G. et al. 2010. Trop Biomed. 27(1): 125-130. PMID: 20562822
4. Hani, H. et al. 2010. Xenotransplantation. 17(6): 469-480. PMID: 21158948

SDS

Datasheet/SDS