Sol8 nuclear extract: sc-2157

Sol8 nuclear extract is sourced from the Sol8 cell line, an immortalized murine myoblast line used primarily to study skeletal muscle differentiation and regeneration. This nuclear extract is rich in transcription factors and other nuclear proteins that are crucial for myogenic differentiation and the repair of muscle tissue. The extract provides a key resource for researchers investigating the activation and function of muscle-specific transcription factors like MyoD and myogenin, which are central to the myogenic regulatory pathway. Utilizing Sol8 nuclear extract, scientists perform electrophoretic mobility shift assays (EMSA) to assess DNA-binding activities, chromatin immunoprecipitation (ChIP) assays to study histone modifications and transcription factor binding at specific gene loci, and co-immunoprecipitation to map protein-protein interactions within the nucleus. These studies are designed to uncover the underlying molecular mechanisms of muscle cell differentiation and to explore the genetic regulation of muscle fiber type specification. Ensuring the provenance and quality of the Sol8 cell line is essential for maintaining the integrity and reliability of the research findings, advancing fundamental biological understanding.

Sol8 nuclear extract References:

1. Sp3 proteins negatively regulate beta myosin heavy chain gene expression during skeletal muscle inactivity.  |  Tsika, G., et al. 2004. Mol Cell Biol. 24: 10777-91. PMID: 15572681
2. Collaborative interactions between MEF-2 and Sp1 in muscle-specific gene regulation.  |  Grayson, J., et al. 1998. J Cell Biochem. 70: 366-75. PMID: 9706874