MEG-01 nuclear extract: sc-2150
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; chronic myelogenous leukemia cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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MEG-01 nuclear extract is derived from the MEG-01 cell line, primarily used in scientific research to investigate the nuclear mechanisms and regulatory processes of megakaryocytes. This extract contains a wide range of nuclear proteins, including transcription factors and regulatory elements, which are crucial for studying gene expression, DNA-protein interactions, and chromatin dynamics. The MEG-01 nuclear extract facilitates a detailed examination of the transcriptional activities specific to megakaryocytic lineage, enabling researchers to dissect the complex regulatory networks that guide megakaryocyte differentiation and platelet formation. In research settings, this extract is particularly valuable for chromatin immunoprecipitation assays, which help identify the binding sites of specific transcription factors and the histone modifications associated with active or repressed gene states. Additionally, it is used in electrophoretic mobility shift assays (EMSA) to assess DNA-binding protein activities within a megakaryocytic context. This type of nuclear extract provides a robust model for understanding the nuclear signaling pathways, focusing purely on the biological and biochemical mechanisms at play in basic cellular research. The origin of the MEG-01 cell line is stringently verified to ensure consistency and reliability in experimental outcomes.
MEG-01 nuclear extract References:
- Transcriptional regulation of cell type-specific expression of the TATA-less A subunit gene for human coagulation factor XIII. | Kida, M., et al. 1999. J Biol Chem. 274: 6138-47. PMID: 10037697
- Human bone marrow megakaryocytes and platelets express PPARgamma, and PPARgamma agonists blunt platelet release of CD40 ligand and thromboxanes. | Akbiyik, F., et al. 2004. Blood. 104: 1361-8. PMID: 15130939
- An intronic enhancer regulates cyclooxygenase-1 gene expression. | DeLong, CJ. and Smith, WL. 2005. Biochem Biophys Res Commun. 338: 53-61. PMID: 16105649
- Differential gene expression, GATA1 target genes, and the chemotherapy sensitivity of Down syndrome megakaryocytic leukemia. | Ge, Y., et al. 2006. Blood. 107: 1570-81. PMID: 16249385
- Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. | Sawada, T., et al. 2008. J Biol Chem. 283: 26820-8. PMID: 18667423
- The leukemia associated ETO nuclear repressor gene is regulated by the GATA-1 transcription factor in erythroid/megakaryocytic cells. | Ajore, R., et al. 2010. BMC Mol Biol. 11: 38. PMID: 20487545
- Molecular mechanisms of bleeding disorderassociated GFI1BQ287* mutation and its affected pathways in megakaryocytes and platelets. | van Oorschot, R., et al. 2019. Haematologica. 104: 1460-1472. PMID: 30655368
- Characterization of a genomic region 8kb downstream of GFI1B associated with myeloproliferative neoplasms. | van Bergen, MGJM., et al. 2021. Biochim Biophys Acta Mol Basis Dis. 1867: 166259. PMID: 34450246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEG-01 nuclear extract | sc-2150 | 250 µg/0.05 ml | $160.00 |