MCF7 nuclear extract: sc-2149

MCF7 nuclear extract is a biochemical tool derived from the MCF7 cell line, extensively utilized in research to study the nuclear processes of breast epithelial cells. This extract is rich in nuclear proteins, including various transcription factors, RNA polymers, and DNA fragments, making it an invaluable resource for examining gene expression and regulatory mechanisms at the nuclear level. The MCF7 nuclear extract is commonly used in applications such as chromatin immunoprecipitation (ChIP) assays to investigate protein-DNA interactions, particularly those involving estrogen receptor signaling pathways. It is also employed in electrophoretic mobility shift assays (EMSA) to study the binding affinity and specificity of nuclear proteins to DNA sequences. These applications contribute to a deeper understanding of the transcriptional regulation in breast epithelial cells, focusing on how cellular processes are governed at the genetic level. The utility of MCF7 nuclear extract in basic biological research is underscored by its role in elucidating the networks of nuclear signaling that influence cell cycle regulation, proliferation, and gene expression. Verification of the MCF7 cell line's origin ensures the extract's reliability and relevance in scientific experiments.

MCF7 nuclear extract References:

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