3611-RF nuclear extract: sc-2143

The 3611-RF nuclear extract is derived from the 3611-RF cell line, which originates from rat fibroblasts. This nuclear extract is a rich source of nuclear proteins, including transcription factors, chromatin-associated proteins, and other regulatory molecules involved in the control of gene expression and nuclear dynamics. By isolating the nuclear contents, this extract provides a focused view of the cellular machinery that operates within the nucleus, offering insights into the regulatory processes that dictate cellular behavior and identity. Researchers utilize the 3611-RF nuclear extract primarily to study the fundamental aspects of nuclear function, such as transcriptional regulation, DNA replication, and repair processes. This extract serves as a crucial tool for probing the interactions between nuclear proteins and DNA, as well as for understanding the changes in nuclear protein composition and activity under various experimental conditions. The use of such extracts in biochemical assays, protein-DNA interaction studies, and gene expression analyses allows scientists to dissect the complex networks of nuclear signaling and regulation. This contributes significantly to the field of molecular biology, helping to explain the mechanisms that underpin cellular responses to internal and external stimuli, and enhancing our understanding of cellular physiology and pathology.

3611-RF nuclear extract References:

1. Inhibition of FOSL1 overexpression in antiestrogen-resistant MCF-7 cells decreases cell growth and increases vacuolization and cell death.  |  Pennanen, PT., et al. 2011. Steroids. 76: 1063-8. PMID: 21570421