C32 + PMA nuclear extract: sc-2137

The C32 + PMA nuclear extract is derived from the C32 cell line, which originates from human melanoma cells, specifically designed to study the effects of melanoma-related signaling pathways. The addition of phorbol 12-myristate 13-acetate (PMA) to these cells is critical as PMA is a known activator of protein kinase C (PKC), a key signaling molecule in various cellular processes including growth, differentiation, and response to external stimuli. This particular nuclear extract contains a wide array of nuclear proteins that are responsive to PKC activation, thus offering a unique perspective into the nuclear events triggered by PKC pathways in melanoma cells. Researchers utilize the C32 + PMA nuclear extract to explore the nuclear mechanisms affected by PKC activation, aiming to understand how these changes influence gene expression, chromatin remodeling, and nuclear signaling in melanoma. The extract provides vital insights into the molecular dynamics within the nucleus following external activation, helping to uncover the layers of regulation that control melanoma cell behavior. This form of research is crucial for deciphering the complex signaling networks that underpin melanoma pathology, excluding direct medical applications but focusing on the fundamental aspects of cellular regulation and signal transduction.