K-562 nuclear extract: sc-2130

K-562 nuclear extract is derived from the K-562 cell line, originally established from a patient with chronic myeloid leukemia and extensively used in research to study hematopoietic and leukemic cell processes. This nuclear extract contains a concentrated array of nuclear proteins, including transcription factors, RNA polymerase, and other regulatory molecules crucial for studying gene expression and signal transduction within leukemic cells. Researchers employ K-562 nuclear extract primarily for its utility in investigating mechanisms of gene regulation involved in cell proliferation and survival. The extract is particularly valuable in chromatin immunoprecipitation (ChIP) assays, where it aids in identifying the interaction sites of specific transcription factors on DNA, as well as in electrophoretic mobility shift assays (EMSA) to study the binding dynamics of nuclear proteins to DNA. These applications allow for a deeper understanding of the transcriptional control mechanisms that are active in hematopoietic malignancies. Additionally, the extract is used to examine the effects of various biochemical modulators on the nuclear components of leukemic cells, thus contributing to fundamental insights into cellular regulation and signaling pathways. The origin and consistency of the K-562 cell line are rigorously verified, ensuring that the extract remains a reliable and relevant tool for basic biological research.

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