A-673 nuclear extract: sc-2128
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; rhabdomyosarcoma cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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The A-673 nuclear extract is sourced from the A-673 cell line, a human cell line established from a malignant rhabdomyosarcoma, a type of soft tissue cancer primarily associated with muscle tissue. This nuclear extract contains a diverse array of nuclear components, including various transcription factors, DNA-binding proteins, and other molecules involved in gene regulation and chromatin structure. These components are integral to studying the underlying mechanisms of gene expression and nuclear behavior in cancer cells. Researchers utilize the A-673 nuclear extract to probe into the cellular and molecular biology of cancer, particularly focusing on the aspects of cellular regulation that are altered in tumor cells. The extract provides a valuable tool for examining the interactions between nuclear proteins and DNA, the effects of various stimuli on nuclear transcriptional activity, and the overall dynamics of the cancer cell nucleus. This research is crucial for understanding the transcriptional deregulation that occurs in cancer, investigating the role of specific nuclear proteins in the progression of malignancies, and exploring the fundamental properties of tumor cell biology. Insights gained from studies using the A-673 nuclear extract contribute significantly to the broader field of cancer research, enhancing our knowledge of tumor biology and the complex interplay of nuclear components in disease states.
A-673 nuclear extract References:
- Alteration of cyclin D1 transcript elongation by a mutated transcription factor up-regulates the oncogenic D1b splice isoform in cancer. | Sanchez, G., et al. 2008. Proc Natl Acad Sci U S A. 105: 6004-9. PMID: 18413612
- PI3K/AKT signaling modulates transcriptional expression of EWS/FLI1 through specificity protein 1. | Giorgi, C., et al. 2015. Oncotarget. 6: 28895-910. PMID: 26336820
- NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth. | Jayabal, P., et al. 2021. Cell Rep. 36: 109254. PMID: 34233189
- MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing. | Vital, T., et al. 2023. Front Oncol. 13: 1099550. PMID: 36793594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-673 nuclear extract | sc-2128 | 250 µg/0.05 ml | $160.00 |