NIH/3T3 + PMA nuclear extract: sc-2125
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- mouse nuclear extract; normal embryo fibroblast cells induced with phorbol
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
QUICK LINKS
SEE ALSO...
The NIH/3T3 + PMA nuclear extract is produced from NIH/3T3 cells, a mouse embryonic fibroblast cell line, stimulated with phorbol 12-myristate 13-acetate (PMA). PMA is a potent activator of protein kinase C (PKC), which plays a critical role in regulating cell differentiation, proliferation, and gene expression. This activation significantly alters the nuclear protein composition, enriching the extract with various transcription factors, co-regulatory proteins, and signaling molecules involved in these processes. In research, this nuclear extract has been instrumental in studying PKC's impact on cellular mechanisms, particularly how it influences transcription factors such as NF-κB and AP-1. These factors are essential for understanding cellular responses to environmental stimuli, including stress response, cell cycle progression, and apoptosis. Additionally, NIH/3T3 + PMA nuclear extracts are used to explore the dynamics of chromatin remodeling and the transcriptional regulation of genes related to cell growth and survival. By providing a complex array of modulated nuclear components, this extract is invaluable for dissecting the signaling pathways and transcriptional networks in basic cell biology research, exclusively focusing on scientific applications.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NIH/3T3 + PMA nuclear extract | sc-2125 | 250 µg/0.05 ml | $160.00 |