A-431 nuclear extract: sc-2122
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; epidermoid carcinoma cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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The A-431 nuclear extract is derived from A-431 cells, a human epidermoid carcinoma cell line extensively utilized in biological research. Originating from epithelial carcinoma, A-431 cells are known for their robust expression of epidermal growth factor receptors (EGFR), making them an excellent model for studying signal transduction and cellular responses to external growth factors. The nuclear extract from these cells includes a rich assortment of nuclear proteins such as transcription factors, regulatory proteins, and components involved in the replication and repair of DNA. These proteins provide a snapshot of the cellular processes occurring within the nucleus, particularly those influenced by EGFR signaling pathways. In research, the A-431 nuclear extract is employed to investigate fundamental aspects of gene expression, nuclear protein interactions, and the mechanisms by which external signals like growth factors influence nuclear dynamics and function. This extract aids in dissecting the pathways that lead to changes in transcriptional activity and chromatin structure, essential for understanding how cells respond to their environment at the molecular level. By using the A-431 nuclear extract, scientists can explore the intricacies of cell signaling, regulation of gene expression, and the overall behavior of cancer cells in response to various stimuli.
A-431 nuclear extract References:
- Ketoconazole suppresses prostaglandin E(2)-induced cyclooxygenase-2 expression in human epidermoid carcinoma A-431 cells. | Kanda, N. and Watanabe, S. 2002. J Invest Dermatol. 119: 174-81. PMID: 12164941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-431 nuclear extract | sc-2122 | 250 µg/0.05 ml | $160.00 |