Western Blotting Luminol Reagent: sc-2048
- Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
- Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
- One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
- Extended exposure times (multiple exposures) to film made possible by long-duration signal
- Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
QUICK LINKS
SEE ALSO...
Luminol reagent, primarily utilized in Western blotting, is a chemiluminescent compound that plays a critical role in detecting proteins. This chemical functions by emitting light upon oxidation, a reaction that is catalyzed by the presence of an oxidizing agent and enhanced in the presence of a catalyst. In the context of Western blotting, luminol is part of the detection system where it reacts with the secondary antibody-conjugated horseradish peroxidase (HRP). This luminescence allows for the precise quantification and visualization of proteins transferred onto a membrane, making it an invaluable tool in molecular biology for protein identification and quantification. Researchers harness this capability to analyze protein expression, post-translational modifications, and protein-protein interactions in various biological samples. Santa Cruz Biotechnology′s Luminol package contains 125 ml each Solution A and Solution B, which combine to form sufficient reagent for a 2,000 cm2 membrane area. For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Western Blotting Luminol Reagent References:
- Stem cell factor and H2O2 induce GLUT1 translocation in M07e cells. | Maraldi, T., et al. 2004. Biofactors. 20: 97-108. PMID: 15322333
- Osteopontin inhibits macrophage nitric oxide synthesis to enhance tumor proliferation. | Wai, PY., et al. 2006. Surgery. 140: 132-40. PMID: 16904962
- Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. | Vostálová, J., et al. 2010. Arch Dermatol Res. 302: 171-81. PMID: 19862537
- Aloe-emodin induces apoptosis of human nasopharyngeal carcinoma cells via caspase-8-mediated activation of the mitochondrial death pathway. | Lin, ML., et al. 2010. Cancer Lett. 291: 46-58. PMID: 19942342
- Activation of mTOR signaling pathway contributes to survival of cervical cancer cells. | Ji, J. and Zheng, PS. 2010. Gynecol Oncol. 117: 103-8. PMID: 20102778
- Leucine promotes leptin receptor expression in mouse C2C12 myotubes through the mTOR pathway. | Mao, X., et al. 2011. Mol Biol Rep. 38: 3201-6. PMID: 20151325
- An economical single-sided antibody incubation method for Western blotting. | Pan, C., et al. 2010. J Virol Methods. 169: 409-11. PMID: 20691216
- Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture. | Zhao, X., et al. 2011. Biotechnol Lett. 33: 1293-300. PMID: 21380526
- Decreased expression of lysosomal alpha-galactosiase A gene in sporadic Parkinson′s disease. | Wu, G., et al. 2011. Neurochem Res. 36: 1939-44. PMID: 21643977
- Screening citrullinated proteins in synovial tissues of rheumatoid arthritis using 2-dimensional western blotting. | Chang, X., et al. 2013. J Rheumatol. 40: 219-27. PMID: 23242181
- Rapid estrogen effects on aromatase phosphorylation in breast cancer cells. | Catalano, S., et al. 2014. Methods Mol Biol. 1204: 155-63. PMID: 25182769
- Modulation of BV-2 microglia functions by novel quercetin pivaloyl ester. | Mrvová, N., et al. 2015. Neurochem Int. 90: 246-54. PMID: 26386394
- Long non-coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway. | Cai, B., et al. 2018. Mol Med Rep. 17: 5477-5483. PMID: 29393477
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Western Blotting Luminol Reagent | sc-2048 | 1 kit | $120.00 |