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home » Specialty Reagents and Buffers » Western Blotting Chemiluminescence Luminol Reagent » Western Blotting Luminol Reagent: sc-2048

Western Blotting Luminol Reagent: sc-2048

  |  Datasheet
  • Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
  • Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
  • One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
  • Extended exposure times (multiple exposures) to film made possible by long-duration signal
  • Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
 
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Product NameCatalog #UnitPriceQtyAddFavorites
Western Blotting Luminol Reagent sc-2048 1 system $114
 For optimal Western Blotting results, most researchers frequently order products  from the following list. (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Acrylamide sc-214492 100 g $49
Acrylamide Solution, 40% sc-3721 1 L $95
Horseradish peroxidase sc-280786 100 mg $146
UltraCruz™ Centrifuge Tube, Conical, 50 ml, racked sc-200252 500 per case $88
UltraCruz™ Wipers, Delicate Task, case sc-3669 60 packs $150
UltraCruz™ Saran wrap sc-3687 100 ft roll $5
UltraCruz™ Autoradiography Film, Blue, 5” x 7” sc-201696 100 sheets $50
UltraCruz™ Autoradiography Film, Blue, 8” x 10” sc-201697 100 sheets $70
UltraCruz™ Autoradiography Tape sc-200214 10 sheets $58
UltraCruz™ Nitrocellulose Pure Transfer Membrane, 0.45 µm, 30 cm x 3 m roll sc-3724 3 m roll $179
UltraCruz™ PVDF Transfer membrane, 0.45 μm, 30 cm x 3 m roll sc-3723 3 m roll $245
Broad Range Markers sc-2361 500 µl $69
Cruz MarkerTM Molecular Weight Standards sc-2035 for 50 gels $101
Western Blotting Luminol Reagent Product Citations
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Western Blotting Luminol Reagent
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sc-2048
ImmunoCruz™ Western Blotting Luminol Reagent: sc-2048
sc-2048
Western Blotting Luminol Reagent: sc-2048. Western blot analysis of Daxx expression in HeLa (A), BJAB (B), Ramos (C), K-562 (D), RAW 264.7 (E) and WEHI-231 (F) whole cell lysates. Antibody tested: Daxx (M-112): sc-7152.
sc-2048
Western Blotting Luminol Reagent: sc-2048. Western blot analysis of AIF expression in AML-193 (A), Jurkat (B) and CTLL-2 (C) whole cell lysates. Antibody tested: AIF (D-20): sc-9416.
sc-2048
Western Blotting Luminol Reagent: sc-2048. Western blot analysis of PKC phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) RAW 264.7 whole cell lysates. Antibodies tested include p-PKC (Thr 507)-R: sc-11770-R (A,B) and PKC (C-20): sc-937 (C,D).
sc-2048
Western Blotting Luminol Reagent: sc-2048. Western blot analysis of Stat1 phosphorylation in untreated (A,C) and IFN-γ-treated (B,D) SK-MEL-28 cell cultures. Lanes A, B and C, D probed, respectively, with Stat1a p91 (C-111): sc-417 (A,B) or with p-Stat1 (Tyr 701): sc-7988 (C,D).
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