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Imidazole (CAS 288-32-4)

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Application:A buffer useful in the pH range of 6.2-7.8
CAS Number:288-32-4
Purity:≥99%
Molecular Weight:68.1
Molecular Formula:C3H4N2
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Imidazole sc-204776 25 g $16
Imidazole sc-204776A 100 g $44
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
Description
Imidazole is useful as a buffer in the pH range of 6.2-7.8 One of the applications of imidazole is in the purification of His-tagged proteins in immobilised metal affinity chromatography(IMAC). Imidazole is used to elute tagged proteins bound to Ni ions attached to the surface of beads in the chromatography column. An excess of imidazole is passed through the column, which displaces the His-tag from nickel co-ordination, freeing the His-tagged proteins. Imidazole has become an important part of many pharmaceuticals. Synthetic imidazoles are present in many fungicides and antifungal, antiprotozoal, and antihypertensive medications. Imidazole is part of the theophylline molecule, found in tea leaves and coffee beans, which stimulates the central nervous system. It is present in the anticancer medication mercaptopurine, which combats leukemia by interfering with DNA activities.
Technical Information
Appearance:White
Physical State:Solid
Solubility:Soluble in water (633 g/L) at 20 °C, acetone, chloroform, pyridine, alcohol, ether. Slightly soluble in benzene. Very slightly soluble in petroleum ether.
Storage:Store at room temperature
Melting Point:87-92 °C
Boiling Point:256 °C
Density:1.03 g/cm3 at 20 °C
Refractive Index:n20D 1.53
IC50:Nitric oxide synthase, inducible: IC50 = 40 µM (Mus musculus); Thromboxane-A synthase: IC50 = 670 µM (human)
Ki Data:Nitric-oxide synthase, brain: Ki= 175 µM (human)
Safety and Reference Information
Transport:UN 3263, Class 8, Packing group III
WGK Germany:1
RTECS:NI3325000
PubChem CID:795
Merck Index:14: 4912
MDL Number:MFCD00005183
EC Number:206-019-2
Beilstein Registry:103853
SMILES:N1C=CN=C1
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
Storrie, B., et al. (1990). Meth. Enzymol. 182, 217.
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