Dihydroethidium (CAS 38483-26-0)

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Application:	An effective dye used to measure oxygen reduction in phagocytes during respiratory burst
CAS Number:	38483-26-0
Purity:	≥95%
Molecular Weight:	315.4
Molecular Formula:	C₂₁H₂₁N₃

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
Dihydroethidium	sc-204724	25 mg	$181
Dihydroethidium	sc-204724A	10 mg	$111

Description

Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H₂0₂ and 0₂^-, and reactive oxygen.

Technical Information

Appearance:	Pink solid
Physical State:	Solid
Solubility:	Soluble in DMSO, DMF, acetonitrile, methanol, and chloroform (20 mg/ml).
Storage:	Store at -20° C
Melting Point:	214.14 °C (Predicted)
Boiling Point:	580.36 °C at 760 mmHg (Predicted)
Density:	1.20 g/cm³ (Predicted)
Refractive Index:	n²⁰_D 1.68 (Predicted)
Fluoresence:	λex 358 nm, λem 461 nm; λex 392 nm, λem 410 nm in methanol

Safety and Reference Information

WGK Germany:	3
RTECS:	SF7935000
PubChem CID:	128682
Beilstein Registry:	5107482
SMILES:	CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Bucana, C.D., et al. 1990. Exp. Cell Res. 190: 69-75. PMID: 2387329
2. Rothe, G. and Valet, G. 1990. J. Leukoc. Biol. 47: 440-448. PMID: 2159514
3. Perticarari, S., et al. 1991. Cytometry. 12: 687-693. PMID: 1782835
4. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55: 253-258. PMID: 8301222
5. Mancini, M., et al. 1997. J. Cell Biol. 138: 449-469. PMID: 9230085
6. Haugen, T.S., et al. 1999. Eur. Respir. J. 14: 1100-1105. PMID: 10596697

SDS

Datasheet/SDS