EDTA, 0.5 M, pH 8.0 (CAS 150-38-9)

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Synonym:	Ethylenediaminetetraacetic Acid Trisodium Salt
Application:	A sterile-filtered solution of 0.5 M EDTA in H2O treated with DEPC, for use in molecular biology apps
CAS Number:	150-38-9
Molecular Weight:	358.19
Molecular Formula:	C₁₀H₁₃N₂O₈Na₃

Description

EDTA, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. Suitable for use in molecular biology applications. EDTA is used extensively as chelator of divalent cations such as Ca²⁺. Useful in molecular biology applications to minimize metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Technical Information

Appearance:	Liquid
Physical State:	Liquid
pH:	8.0
Solubility:	Soluble in water.
Storage:	Store at room temperature
Boiling Point:	614.2 °C at 760 mmHg (Predicted)

Safety and Reference Information

PubChem CID:	9008
Merck Index:	14: 3517
MDL Number:	MFCD00149685
Beilstein Registry:	3858591
SMILES:	C(CN(CC(=O)[O-])CC(=O)[O-])N(CC(=O)O)CC(=O)[O-].[Na+].[Na+].[Na+]

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Child, G.P. 1951. Science. 114: 466-467. PMID: 14892768
2. Friess, E.G., et al. 1954. Arch Biochem. 53: 311-313. PMID: 13208306
3. Sapeika, N. 1955. S. Afr. Med. J. 29: 137-139. PMID: 14358876
4. Sheppard, R.L., et al. 1997. Anal. Chem. 69: 477A-480A. PMID: 9253241
5. Lanigan, R.S. and Yamarik, T.A. 2002. Int. J. Toxicol. 21: 95-142. PMID: 12396676

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