Calcein-AM (CAS 148504-34-1)

5 5 (Based on popularity) 1

Application:	A non-fluorescent probe that can be hydrolyzed to a fluorescent molecule
CAS Number:	148504-34-1
Purity:	≥95%
Molecular Weight:	994.9
Molecular Formula:	C₄₆H₄₆N₂O₂₃

Description

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. The probe can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein as a cytosolic fluorophore. Has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.

Technical Information

Appearance:	Off-white
Physical State:	Solid
Solubility:	Soluble in DMSO (10 mg/ml), and water.
Storage:	Store at -20° C
Boiling Point:	982.73 °C at 760 mmHg (Predicted)
Density:	1.49 g/cm³ (Predicted)
Refractive Index:	n²⁰_D 1.61 (Predicted)
Fluoresence:	λex 496 nm, λem 516 nm in 0.1 M Tris pH 8.0

Safety and Reference Information

WGK Germany:	3
PubChem CID:	390986
MDL Number:	MFCD05861516
SMILES:	CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O) OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Feller, N., et al. 1995. Br. J. Cancer. 72: 543-549. PMID: 7669559
2. Feller, N., et al. 1995. FEBS Lett. 368: 385-388. PMID: 7628644
3. Essodaigui, M., et al. 1998. Biochemistry. 37: 2243-2250. PMID: 9485370
4. Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta. 1366: 177-196. PMID: 9714796
5. Dhar, S., et al. 1998. Eur. J. Pharmacol. 346: 315-322. PMID: 9652375

SDS

Datasheet/SDS