Sudan III (CAS 85-86-9)

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Synonym:	Solvent Red 23
Application:	A lysochrome (fat soluble dye) predominantly used for staining triglycerides in animal tissues (frozen sections)
CAS Number:	85-86-9
Molecular Weight:	352.39
Molecular Formula:	C₂₂H₁₆N₄O

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
Sudan III	sc-203761	25 g	$78
Sudan III	sc-203761B	100 g	$200

Description

Sudan III is predominantly used for staining triglycerides in animal tissues (frozen sections). Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. With the use of certain solvents, may also be used to stain some protein bound lipids in paraffin sections. Sudan I (sc-215922), Sudan III (sc-203761), and Sudan IV (sc-203762) have been classified as category 3 carcinogens by the International Agency for Research on Cancer.

Usage

• A saturated solution in isopropanol is prepared for use as a fat stain in animal tissue. Counterstain for 5 minutes in acid alum hematoxylin (approx. 0.1%). Fats will appear yellow orange in color and the nuclei will appear blue. The cytoplasm will appear green.
• Since lysochromes are soluble in non polar solvents, including xylene and toluene, and also in ethanol, these fluids must not be put on to sections after fats have been stained or the dye will be dissolved out; therefore resinous mounting media are not recommended. We recommend mounting sections with Crystal Mounting Medium; sc-24943.

Technical Information

Appearance:	Brown to dark red-brown powder
Physical State:	Solid
Solubility:	Soluble in ether, acetone, chloroform, benzene, ethanol (slightly soluble), DMSO, Hot glacial acetic acid (Slightly), hot glycerol, and toluene. Insoluble in water, and Lye.
Storage:	Store at room temperature
Melting Point:	199 °C (lit.)(dec.)
Boiling Point:	584.60 °C at 760 mmHg (Predicted)
Density:	1.22 g/cm³ (Predicted)
Refractive Index:	n²⁰_D 1.66 (Predicted)
Color Index Number:	26100

Safety and Reference Information

WGK Germany:	3
RTECS:	QK4250000
PubChem CID:	6789251
Merck Index:	14: 8884
MDL Number:	MFCD00003905
EC Number:	201-638-4
Beilstein Registry:	931185
SMILES:	C1=CC=C(C=C1)N=NC2=CC=C(C=C2)N/N=C\3/C(=O)C=CC4=CC=CC=C43

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Kodama, H., et al. 1977. J. Dermatol. 4: 41-48. PMID: 15461324
2. Masamune, O., et al. 1977. Tohoku J. Exp. Med. 122: 397-402. PMID: 72437
3. Yoshimura, S., et al. 1980. Acta Cytol. 24: 44-48. PMID: 6153489
4. Refat, N.A., et al. 2008. J. Biochem. Mol. Toxicol. 22: 77-84. PMID: 18418879

SDS

Datasheet/SDS