Pyronin Y (CAS 92-32-0)

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Application:	A dye that intercalates and stains RNA red
CAS Number:	92-32-0
Molecular Weight:	302.8
Molecular Formula:	C₁₇H₁₉ClN₂O

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
Pyronin Y	sc-203755	1 g	$28
Pyronin Y	sc-203755A	5 g	$85

This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.

Description

Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrug-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide (sc-203947). Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G₁ phase, whereas at high concentrations the cells arrest is in the G₂ and S phase. Pyronin Y has been used in combination with Alcian Blue (sc-214517) to asses and study pulmonary diseases, and in combination with Methyl Green (sc-215348) to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry.

Technical Information

Appearance:	Green powder
Physical State:	Solid
Solubility:	Soluble in water (30 mg/mL), methyl cellosolve (9 mg/mL), and ethanol (3 mg/mL).
Storage:	Store at room temperature
Melting Point:	250-260 °C (lit.)
Density:	0.64 g/cm³ at 20
Fluoresence:	λex 546 nm, λem 565 nm in DMSO
IC50:	HEK293 (Embryonic kidney fibroblasts): IC₅₀ = 4540 nM (human)

Safety and Reference Information

Transport:	UN 3335, Class 9, Packing group n/a
WGK Germany:	3
RTECS:	BQ1450000
PubChem CID:	7085
Merck Index:	14: 8007
MDL Number:	MFCD00011725
EC Number:	202-147-8
Beilstein Registry:	3795789
SMILES:	CN(C)C1=CC2=C(C=C1)C=C3C=CC(=[N+](C)C)C=C3O2.[Cl-]

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Crissman, H.A., et al. 1985. J. Cell Biol. 101: 141-147. PMID: 2409095
2. Darzynkiewicz, Z., et al. 1986. Cancer Res. 46: 5760-5766. PMID: 2428484
3. Li, B., et al. 2002. Histochem. J. 34: 299-303. PMID: 12769261
4. Prentø, P. and Lyon, H.O. 2003. Biotech Histochem. 78: 27-33. PMID: 12713139
5. Chusri, S., et al. 2009. J. Antimicrob. Chemother. 64: 1203-1211. PMID: 19861335
6. Karpińska-Kaczmarczyk, K., et al. 2009. Pol J Pathol. 60: 124-129. PMID: 20069505
7. Meyerholz, D.K., et al. 2009. Vet. Pathol. 46: 325-328. PMID: 19261646

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