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Cruz Marker™ Molecular Weight Standards: sc-2035 |
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- Recommended use is 2-4 µl per lane in SDS-PAGE
- Each vial contains 200 µl volume, sufficient for 50 gels
- The ladder consists of six bands which appear on the final Western blot film: 132 kDa, 90 kDa, 55 kDa, 43 kDa, 34 kDa, 23 kDa
- These reagents must be used in combination with Cruz Marker™ compatible Western blotting secondary antibodies available only from Santa Cruz Biotechnology, Inc.
- Cruz Marker™ Molecular Weight Standards appear only on the final Western blot film after incubation with Cruz Marker™ compatible Western blotting secondary antibodies. These are not prestained markers.
- Cruz Marker™ Molecular Weight Standards are provided ready to use in SDS-PAGE loading buffer and can be loaded directly into an SDS-PAGE gel. No dilution or reconstitution is required
- Cruz Markers™ are provided for use as internal molecular weight standards for chemiluminescence Western blotting applications and are ideal for approximating the molecular weight of target proteins.
- Cruz Marker™ Molecular Weight Standards use a proprietary technology to allow researchers to visualize the final molecular weight ladder of six bands clearly and consistently on the final Western blot film. Ideal for use in combination with our Prestained Molecular Weight Markers (Broad, High and Low range: sc-2361, sc-2362, sc-2360) for verification and calibration of protein standard migration in SDS PAGE gels and Western blotting membranes.
- These reagents must be used in combination with Cruz Marker™ compatible Western blotting secondary antibodies available only from Santa Cruz Biotechnology, Inc.
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Cruz Marker™ Molecular Weight Standards
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Cruz Marker TM molecular weight standards showing total marker panel (A) and individual 132 kDa (B), 90 kDa (C), 55 kDa (D), 43 kDa (E), 34 kDa (F) and 23 kDa (G) markers using Cruz Marker TM Western blotting secondary antibody: sc-2031.
Western blot analysis of Stat1 phosphorylation in untreated (A,C) and IFN-γ-treated (B,D) SK-MEL-28 cell cultures. Lanes A, B and C, D probed, respectively, with Stat1α p91 (C-111): sc-417 (A,B) or with p-Stat1 (Tyr 701): sc-7988 (C,D).
Western blot analysis of Daxx expression in HeLa (A), BJAB (B), Ramos (C), K-562 (D), RAW 264.7 (E) and WEHI-231 (F) whole cell lysates. Antibody tested: Daxx (M-112): sc-7152.
Western blot analysis of AIF expression in AML-193 (A), Jurkat (B) and CTLL-2 (C) whole cell lysates. Antibody tested: AIF (D-20): sc-9416.
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