Guanidine Thiocyanate (CAS 593-84-0)
See product citations (5)
QUICK LINKS
Guanidine Thiocyanate is a chaotropic agent used in molecular biology research for the extraction of nucleic acids. Its high efficacy in denaturing proteins makes it particularly valuable in the isolation of RNA, as it helps to inactivate RNases that would otherwise degrade the RNA during the extraction process. Guanidine Thiocyanate is a critical component in the preparation of lysates from cells and tissues where it disrupts cellular structures, thereby releasing RNA into solution. In addition to its role in nucleic acid extraction, this chemical is used to study protein folding and to denature proteins in the study of their primary structure. Furthermore, Guanidine Thiocyanate is used in the creation of density gradients for the purification of nucleic acids, allowing for the separation of RNA from DNA and other cellular components.
Guanidine Thiocyanate (CAS 593-84-0) References
- Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture. | Jacobsen, N., et al. 2004. Nucleic Acids Res. 32: e64. PMID: 15096560
- [Retention of nucleic acid integrity in guanidine thiocyanate lysates of whole blood]. | Kravchenko, AV., et al. 2006. Bioorg Khim. 32: 609-14. PMID: 17180911
- Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate. | Ding, LW., et al. 2008. Anal Biochem. 374: 426-8. PMID: 18166144
- Distinct effects of guanidine thiocyanate on the structure of superfolder GFP. | Stepanenko, OV., et al. 2012. PLoS One. 7: e48809. PMID: 23144981
- The folding of staphylococcal nuclease in the presence of methanol or guanidine thiocyanate. | Nakano, T. and Fink, AL. 1990. J Biol Chem. 265: 12356-62. PMID: 2373696
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. | Chomczynski, P. and Sacchi, N. 1987. Anal Biochem. 162: 156-9. PMID: 2440339
- Extraction of peptides from guanidine thiocyanate homogenates used to isolate RNA. | Iadarola, MJ. and Naranjo, JR. 1988. Peptides. 9: 669-71. PMID: 2458576
- A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation. | Emadi, S. and Behzadi, M. 2014. Biochem Biophys Res Commun. 450: 1339-44. PMID: 25003319
- A single protocol for extraction of gDNA from bacteria and yeast. | Vingataramin, L. and Frost, EH. 2015. Biotechniques. 58: 120-5. PMID: 25757544
- Environmentally friendly method of RNA isolation. | Drygin, YF., et al. 2021. Anal Biochem. 620: 114113. PMID: 33524410
- Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials. | Honeywood, MJ., et al. 2021. J Virol Methods. 297: 114262. PMID: 34384823
- Processing Polyelectrolyte Complexes with Deep Eutectic Solvents. | Chen, Y. and Shull, KR. 2021. ACS Macro Lett. 10: 1243-1247. PMID: 35549044
- Two-dimensional polyacylamide gel electrophoresis of envelope proteins of Escherichia coli. | Johnson, WC., et al. 1975. Appl Microbiol. 29: 405-13. PMID: 803821
- Direct solution hybridization of guanidine thiocyanate-solubilized cells for quantitation of mRNAs in hepatocytes. | Kaabache, T., et al. 1995. Anal Biochem. 232: 225-30. PMID: 8747479
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Guanidine Thiocyanate, 100 g | sc-202638 | 100 g | $89.00 | |||
Guanidine Thiocyanate, 500 g | sc-202638A | 500 g | $215.00 | |||
Guanidine Thiocyanate, 1 kg | sc-202638B | 1 kg | $310.00 | |||
Guanidine Thiocyanate, 25 kg | sc-202638C | 25 kg | $4296.00 |