A cell-permeable carbamate compound that acts as a potent, selective, and irreversible inhibitor of fatty acid amide hydrolase (FAAH; IC50 = 4.6 nM in brain membranes). Shown to block anandamide breakdown in rat cortical neurons (IC50 = 500 pM) and modulate anxiety in rats (ID50 = 0.15 mg/Kg). Does not affect the activities of several serine hydrolases (IC50 > 30 µM), including AChE (acetylcholinesterase), BCh (butyrylcholinesterase) and MGL (monoglyceride lipase). It also does not interfere with the binding of anandamide to cannabinoid receptors CB1 and CB2 (IC50 > 100 µM), as well as several ion-channels and neurotransmitter transporters.
