Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (CAS 23491-45-4)

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride is a fluorescent dye that belongs to the bisbenzimide family of compounds, characterized by its planar, heterocyclic structure and high degree of conjugation. This fluorochrome exhibits a strong affinity for binding to DNA, particularly to the minor groove of double-stranded DNA, where it undergoes a process called fluorescence resonance energy transfer (FRET). Upon binding, it absorbs energy and transfers it to a nearby molecule, leading to the emission of light at a longer wavelength, typically in the blue-violet spectrum. Researchers have leveraged this property to utilize Bisbenzimide H 33258 Fluorochrome, Trihydrochloride as a fluorescent probe to investigate various biological processes, including DNA structure and gene expression. The binding mechanism of this fluorochrome is highly specific, allowing it to target specific DNA sequences and structures, making it a useful tool for understanding DNA-protein interactions and chromatin organization